Živković Lada, Borozan Sunčica, Čabarkapa Andrea, Topalović Dijana, Ciptasari Ummi, Bajić Vladan, Spremo-Potparević Biljana
Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
Oxid Med Cell Longev. 2017;2017:8759764. doi: 10.1155/2017/8759764. Epub 2017 Feb 21.
The ability of mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against HO-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of on the interventional level, respectively, after treatment. in concentration of 250 g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in HO induced DNA damage 15 min after the application of , reaching the maximum potency after 30 min. Analysis of antioxidant properties of revealed strong OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of and its strong OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
首次评估了干燥的粉末状菌丝体形式的蘑菇的抗基因毒性特性。通过彗星试验，在预处理和后处理方案中检测了其对人外周血细胞中HO诱导的DNA损伤的抗基因毒性作用。结果表明，在治疗后，分别在干预水平上具有更好的抗基因毒性特性。治疗后浓度为250μg/mL的该物质对DNA损伤的作用最为有效。修复动力学评估显示，在应用该物质后15分钟，HO诱导的DNA损伤减少，30分钟后达到最大效力。对该物质抗氧化特性的分析表明，其具有较强的羟基清除能力和中等的还原能力，而其DPPH清除能力较弱。关于我们的研究结果，我们可以得出结论，我们的初步结果证明了该物质的抗基因毒性特性及其较强的羟基清除能力。其特性背后的机制应在体内研究中进一步评估。
