在健康人群和基层医疗患者中筛查产MCR的粪便携带情况。
Screening for fecal carriage of MCR-producing in healthy humans and primary care patients.
作者信息
Zurfluh Katrin, Stephan Roger, Widmer Andreas, Poirel Laurent, Nordmann Patrice, Nüesch Hans-Jakob, Hächler Herbert, Nüesch-Inderbinen Magdalena
机构信息
Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland.
Division of Infectious Diseases and Hospital Epidemiology, University of Basel, 4031 Basel, Switzerland.
出版信息
Antimicrob Resist Infect Control. 2017 Mar 14;6:28. doi: 10.1186/s13756-017-0186-z. eCollection 2017.
BACKGROUND
The extent of the occurrence of the plasmid-encoded colistin resistance genes and among humans is currently sparsely studied in Western Europe.
OBJECTIVES
To determine the occurrence of MCR-producing in fecal samples of healthy humans with high occupational exposure to food and primary care patients in Switzerland.
METHODS
Stool samples from 1091 healthy individuals and fecal swabs from 53 primary care patients were screened for polymyxin-resistant using LB agar containing 4 mg/L colistin. Minimal inhibitory concentrations (MICs) of colistin were determined for non-intrinsic colistin-resistant isolates. Isolates were screened by PCR for the presence of and genes.
RESULTS
The fecal carriage rate of colistin resistant (MIC value >2 mg/l) was 1.5% for healthy people and 3.8% for primary care patients. Isolates included ( = 9), ( = 3), ( = 4), ( = 1) and ( = 1). None of the isolates harbored the or genes.
CONCLUSIONS
There is no evidence for the presence of MCR-producers in the fecal flora of healthy people or primary care patients. Therefore, the risk of transfer of genes from animals, food or the environment to humans is likely to be low in Switzerland.
背景
在西欧，目前对质粒编码的黏菌素耐药基因在人类中的发生程度研究较少。
目的
确定瑞士食品行业职业暴露高的健康人群和初级保健患者粪便样本中产MCR细菌的发生率。
方法
使用含4mg/L黏菌素的LB琼脂对1091名健康个体的粪便样本和53名初级保健患者的粪便拭子进行耐多黏菌素筛选。对非固有耐黏菌素分离株测定黏菌素的最低抑菌浓度（MIC）。通过PCR筛选分离株中是否存在mcr-1和mcr-2基因。
结果
健康人群中耐黏菌素（MIC值>2mg/l）的粪便携带率为1.5%，初级保健患者为3.8%。分离株包括肺炎克雷伯菌（n = 9）、大肠埃希菌（n = 3）、产气肠杆菌（n = 4）、阴沟肠杆菌（n = 1）和弗劳地枸橼酸杆菌（n = 1）。所有分离株均未携带mcr-1或mcr-2基因。
结论
没有证据表明健康人群或初级保健患者的粪便菌群中存在产MCR细菌。因此，在瑞士，mcr基因从动物、食物或环境转移到人类的风险可能较低。
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