Nordhoff Katja, Scharlach Martina, Effelsberg Natalie, Knorr Carolin, Rocker Dagmar, Claussen Katja, Egelkamp Richard, Mellmann Alexander C, Moss Andreas, Müller Ilona, Roth Sarah Andrea, Werckenthin Christiane, Wöhlke Anne, Ehlers Joachim, Köck Robin
Lower Saxony State Office for Consumer Protection and Food Safety (LAVES), Oldenburg, Germany.
Perioperative Inflammation and Infection, Department of Human Medicine, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany.
Front Microbiol. 2023 Jun 2;14:1183984. doi: 10.3389/fmicb.2023.1183984. eCollection 2023.
The emergence of carbapenem-resistant bacteria causing serious infections may lead to more frequent use of previously abandoned antibiotics like colistin. However, mobile colistin resistance genes () can jeopardise its effectiveness in both human and veterinary medicine. In Germany, turkeys have been identified as the food-producing animal most likely to harbour -positive colistin-resistant Enterobacterales (-Col-E). Therefore, the aim of the present study was to assess the prevalence of both -Col-E and carbapenemase-producing Enterobacterales (CPE) in German turkey herds and humans in contact with these herds.
In 2018 and 2019, 175 environmental (boot swabs of turkey faeces) and 46 human stool samples were analysed using a combination of enrichment-based culture, PCR, core genome multilocus sequence typing (cgMLST) and plasmid typing.
-Col-E were detected in 123 of the 175 turkey farms in this study (70.3%). -Col-E isolates were (98.4%) and spp. (1.6%). Herds that had been treated with colistin were more likely to harbour -Col-E, with 82.2% compared to 66.2% in untreated herds ( = 0.0298). Prevalence also depended on husbandry, with 7.1% -Col-E in organic farms compared to 74.5% in conventional ones ( < 0.001). In addition, four of the 46 (8.7%) human participants were colonised with -Col-E. -Col-E isolates from stables had minimum inhibitory concentrations (MICs) from 4 to ≥ 32 mg/l, human isolates ranged from 4 to 8 mg/l. cgMLST showed no clonal transmission of isolates. For one farm, plasmid typing revealed great similarities between plasmids from an environmental and a human sample. No CPE were found in turkey herds or humans.
These findings confirm that -Col-E-prevalence is high in turkey farms, but no evidence of direct zoonotic transmission of clonal -Col-E strains was found. However, the results indicate that plasmids may be transmitted between isolates from animals and humans.
产碳青霉烯酶细菌引发严重感染的出现，可能导致更频繁地使用如黏菌素这类先前已被弃用的抗生素。然而，可移动的黏菌素耐药基因（）可能会危及它在人类医学和兽医学中的有效性。在德国，火鸡已被确定为最有可能携带阳性黏菌素耐药肠杆菌（-Col-E）的产肉动物。因此，本研究的目的是评估德国火鸡群以及与这些鸡群有接触的人群中-Col-E和产碳青霉烯酶肠杆菌（CPE）的流行情况。
在2018年和2019年，使用基于富集培养、聚合酶链反应（PCR）、核心基因组多位点序列分型（cgMLST）和质粒分型相结合的方法，对175份环境样本（火鸡粪便靴拭子）和46份人类粪便样本进行了分析。
在本研究的175个火鸡养殖场中，有123个（70.3%）检测到-Col-E。-Col-E分离株主要是（98.4%）和 spp.（1.6%）。使用过黏菌素治疗的鸡群更有可能携带-Col-E，比例为82.2%，而未治疗鸡群的这一比例为66.2%（ = 0.0298）。流行情况还取决于养殖方式，有机农场中-Col-E的比例为7.1%，而传统农场中为74.5%（< 0.001）。此外，46名人类参与者中有4名（8.7%）被-Col-E定植。来自鸡舍的-Col-E分离株的最低抑菌浓度（MIC）为4至≥32毫克/升，人类分离株的MIC范围为4至8毫克/升。cgMLST显示分离株无克隆传播。对于一个养殖场，质粒分型显示环境样本和人类样本中的质粒有很大相似性。在火鸡群或人类中未发现CPE。
这些发现证实火鸡养殖场中-Col-E的流行率很高，但未发现克隆-Col-E菌株直接人畜共患传播的证据。然而，结果表明质粒可能在动物和人类的分离株之间传播。
