Kuncham Ramprasad, Sivaprakasam Thiyagarajan, Puneeth Kumar R, Sreenath P, Nayak Ravi, Thayumanavan Tha, Subba Reddy Gopireddy V
Eurofins Genomics India Pvt. Ltd, Bengaluru 560048, Karnataka, India.
School of Biotechnology, Dr. G.R. Damodaran College of Science, Coimbatore 641014, Tamilnadu, India.
Genom Data. 2017 Mar 3;12:44-48. doi: 10.1016/j.gdata.2017.03.001. eCollection 2017 Jun.
Chironomid larvae that inhabit in aquatic sediments play an important role as vector for bacterial pathogens. Its life cycle consists of four stages i.e. eggs, larvae, pupae and adult. In the present study we identified bacterial species associated with whole larvae of chironomids from 11 lake sediments of Bangalore region using 16s rRNA gene Sanger sequencing. We found that larvae from all lake sediments associated with bacterial species which include key pathogens. Totally we identified 65 bacterial isolates and obtained GenBank accession numbers (KX980423 - KX980487). Phylogenetic tree constructed using MEGA 7 software and tree analysis highlight the predominant bacterial community associated with larvae which include (43.08%; 28 isolates) and (24.62%; 16 isolates), , , (6.15%; 4 isolates each), (4.62%; 3 isolates) and (3.08%; 2 isolates). Current findings state that among bacterial population , and with serotypes are commonly associated with larvae in maximum lake points. In other hand , , , , , and other bacterial species were identified moderately in all lakes. Interestingly, we identified first time Gram negative, rod shaped pathogenic organism of and Gram negative, rod shaped organism associating chironomid larvae.
栖息于水底沉积物中的摇蚊幼虫作为细菌病原体的传播媒介发挥着重要作用。其生命周期包括四个阶段，即卵、幼虫、蛹和成虫。在本研究中，我们使用16s rRNA基因桑格测序法，从班加罗尔地区11个湖泊沉积物中的摇蚊幼虫整体中鉴定出相关细菌种类。我们发现，来自所有湖泊沉积物的幼虫都与包括关键病原体在内的细菌种类有关。我们总共鉴定出65株细菌分离株，并获得了GenBank登录号（KX980423 - KX980487）。使用MEGA 7软件构建的系统发育树和树状分析突出了与幼虫相关的主要细菌群落，其中包括（43.08%；28株分离株）和（24.62%；16株分离株）， 、 、 （各6.15%；4株分离株）， （4.62%；3株分离株）和 （3.08%；2株分离株）。目前的研究结果表明，在细菌种群中，具有血清型的 、 和 通常在大多数湖泊点与幼虫相关。另一方面， 、 、 、 、 和其他细菌种类在所有湖泊中被中度鉴定。有趣的是，我们首次鉴定出与摇蚊幼虫相关的革兰氏阴性、杆状致病生物 和 革兰氏阴性、杆状生物。
