Department of Biology, Faculty of Science and Science Education, University of Haifa, Oranim, Tivon 36006, Israel.
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3025-9. doi: 10.1099/ijs.0.007211-0. Epub 2009 Jul 30.
A Gram-negative, aerobic, chemo-organotrophic, catalase- and oxidase-positive strain, designated AIMA4(T), was isolated from a chironomid (Diptera; Chironomidae) egg mass which was sampled from a waste stabilization pond in northern Israel. Phylogenetic analysis based on the 16S rRNA gene placed strain AIMA4(T) in the genus Brachymonas (class Betaproteobacteria). Strain AIMA4(T) shared 16S rRNA gene sequence similarity values of 96.9 % with Brachymonas denitrificans JCM 9216(T) and <95.7 % with the type strains of other genera. Strain AIMA4(T) was found to be a non-motile coccobacillus or rod that did not grow under anaerobic conditions and did not produce acid from carbohydrates. Growth was observed at salinities from 0 to 2.5 % (w/v) NaCl, at pH values from 5.0 to 9.0 and at temperatures from 18 to 37 degrees C. The major fatty acids (>10 %) of strain AIMA4(T) were C(16 : 1)omega7c, C(16 : 0) and C(18 : 1)omega7c. Phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine were the predominant components of the polar lipids. The major ubiquinone was Q-8. On the basis of phenotypic properties and phylogenetic distinctiveness, strain AIMA4(T) (=LMG 24400(T)=DSM 19884(T)) was classified in the genus Brachymonas as the type strain of a novel species for which the name Brachymonas chironomi sp. nov. is proposed.
一株革兰氏阴性、需氧、化能有机营养型、接触酶和氧化酶阳性的菌株，被命名为 AIMA4(T)，从以色列北部一个废物稳定池中采集的摇蚊（双翅目；摇蚊科）卵块中分离得到。基于 16S rRNA 基因的系统发育分析将菌株 AIMA4(T)归入短杆菌属（β变形菌纲）。菌株 AIMA4(T)与 Brachymonas denitrificans JCM 9216(T)的 16S rRNA 基因序列相似性为 96.9%，与其他属的模式菌株的相似性<95.7%。菌株 AIMA4(T)被发现是一种非运动性的球菌或杆菌，不能在厌氧条件下生长，也不能从碳水化合物中产生酸。在 0 到 2.5%（w/v）NaCl 的盐度、pH 值为 5.0 到 9.0 和温度为 18 到 37 摄氏度的条件下观察到生长。菌株 AIMA4(T)的主要脂肪酸（>10%）为 C(16:1)omega7c、C(16:0)和 C(18:1)omega7c。磷脂酰乙醇胺、磷脂甘油和磷脂酰丝氨酸是极性脂的主要成分。主要的醌类是 Q-8。基于表型特征和系统发育的独特性，菌株 AIMA4(T)（=LMG 24400(T)=DSM 19884(T)）被分类为短杆菌属的一个新种的模式菌株，该新种被命名为 Brachymonas chironomi sp. nov.。
