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经体外胃肠道消化的巴西棕榈果实(Euterpe edulis Martius)生物活性化合物的生物可及性及抗氧化潜力

Bioaccessibility of bioactive compounds and antioxidant potential of juçara fruits (Euterpe edulis Martius) subjected to in vitro gastrointestinal digestion.

作者信息

Schulz Mayara, Biluca Fabíola Carina, Gonzaga Luciano Valdemiro, Borges Graciele da Silva Campelo, Vitali Luciano, Micke Gustavo Amadeu, de Gois Jefferson Santos, de Almeida Tarcisio Silva, Borges Daniel Lazaro Gallindo, Miller Paul Richard Momsen, Costa Ana Carolina Oliveira, Fett Roseane

机构信息

Department of Food Science and Technology, Federal University of Santa Catarina, Florianopolis 88034-001, Brazil.

Department of Food Science and Technology, Federal University of Santa Catarina, Florianopolis 88034-001, Brazil.

出版信息

Food Chem. 2017 Aug 1;228:447-454. doi: 10.1016/j.foodchem.2017.02.038. Epub 2017 Feb 10.

Abstract

An in vitro method involving simulated gastrointestinal digestion was used to assess the bioaccessibility of fifteen minerals, twenty-two phenolic compounds and the antioxidant capacity in juçara fruit during seven ripening stages. For minerals and phenolics, respectively, initial contents were up to 1325.9 and 22.9mg100g, whereas after in vitro digestion, the maximum values were 556.7 and 14.43mg100g (dry matter). Antioxidant capacity, determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) and ferric reducing antioxidant power (FRAP), after in vitro digestion decreased 51-78% when compared to the crude extract. Bioaccessible fractions of quercetin, protocatechuic and p-coumaric acids presented positive and significant correlation with results of DPPH and FRAP. Furthermore, our study demonstrated that the ripening stages of juçara fruit influenced the bioaccessibility of compounds and antioxidant capacity, which presented higher levels in purple fruits collected 42-69days after the appearance of the red berries on bunches.

摘要

采用一种涉及模拟胃肠消化的体外方法,评估了七个成熟阶段的巴西棕榈果实中15种矿物质、22种酚类化合物的生物可及性以及抗氧化能力。矿物质和酚类物质的初始含量分别高达1325.9和22.9mg/100g,而体外消化后,最大值分别为556.7和14.43mg/100g(干物质)。通过2,2-二苯基-1-苦基肼自由基清除活性(DPPH)和铁还原抗氧化能力(FRAP)测定的抗氧化能力,体外消化后与粗提物相比降低了51-78%。槲皮素、原儿茶酸和对香豆酸的生物可及部分与DPPH和FRAP的结果呈正相关且具有显著性。此外,我们的研究表明,巴西棕榈果实的成熟阶段影响了化合物的生物可及性和抗氧化能力,在果串上出现红色浆果后42-69天采集的紫色果实中这些指标呈现出较高水平。

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