Rowland Institute at Harvard University, 100 Edwin H. Land Blvd., Cambridge, MA 02142, USA.
Massachusetts General Hospital, Charlestown, MA 02129, USA.
Sci Rep. 2017 Mar 20;7:44757. doi: 10.1038/srep44757.
Successful developments of new therapeutic strategies often rely on the ability to deliver exogenous molecules into cytosol. We have developed a versatile on-chip vortex-assisted electroporation system, engineered to conduct sequential intracellular delivery of multiple molecules into various cell types at low voltage in a dosage-controlled manner. Micro-patterned planar electrodes permit substantial reduction in operational voltages and seamless integration with an existing microfluidic technology. Equipped with real-time process visualization functionality, the system enables on-chip optimization of electroporation parameters for cells with varying properties. Moreover, the system's dosage control and multi-molecular delivery capabilities facilitate intracellular delivery of various molecules as a single agent or in combination and its utility in biological research has been demonstrated by conducting RNA interference assays. We envision the system to be a powerful tool, aiding a wide range of applications, requiring single-cell level co-administrations of multiple molecules with controlled dosages.
新治疗策略的成功发展往往依赖于将外源分子递送到细胞质中的能力。我们开发了一种通用的片上涡旋辅助电穿孔系统，旨在以低电压、剂量控制的方式将多种分子递送到各种细胞类型的细胞内。微图案化平面电极允许大幅度降低操作电压，并与现有的微流控技术无缝集成。该系统配备实时过程可视化功能，可针对具有不同特性的细胞在片上优化电穿孔参数。此外，该系统的剂量控制和多分子传递能力可促进各种分子作为单一试剂或组合的细胞内传递，并且通过进行 RNA 干扰测定已经证明了其在生物研究中的实用性。我们设想该系统将成为一种强大的工具，辅助广泛的应用，需要以受控剂量对多种分子进行单细胞水平的共同给药。
