一项针对商业和12种内部实时聚合酶链反应检测方法检测肺炎支原体的多中心评估。
Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae.
作者信息
Dumke Roger, Benitez Alvaro J, Chalker Victoria, Gullsby Karolina, Henrich Birgit, Hidalgo-Grass Carlos, Hoogenboezem Theo, Kese Darja, Loens Katherine, Maaskant Jolanda, Michael-Gayego Ayelet, Moses Allon E, Nir-Paz Ran, Pas Suzan D, Pereyre Sabine, Petersen Randi F, Rosenblatt Meike, van Rossum Annemarie M C, Uldum Søren A, Unger Wendy W J, Ursi Dominique, Winchell Jonas M, Bebear Cecile
机构信息
TU Dresden, Dresden, Germany.
Centers for Disease Control and Prevention, Atlanta, GA, USA.
出版信息
Diagn Microbiol Infect Dis. 2017 Jun;88(2):111-114. doi: 10.1016/j.diagmicrobio.2017.03.004. Epub 2017 Mar 8.
Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.
通过实时聚合酶链反应检测肺炎支原体在各实验室尚未标准化。我们实施了一项标准化方案，以比较13种商业和内部方法的性能。尽管样本阈值存在差异，但所有检测方法每次反应均能检测到至少20个肺炎支原体基因组。
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