TU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany
TU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany.
J Clin Microbiol. 2014 Nov;52(11):4078-81. doi: 10.1128/JCM.02048-14. Epub 2014 Sep 10.
Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.
四种用于检测肺炎支原体的商业实时 PCR 检测方法进行了测试,并将结果与内部方法的结果进行了比较。尽管检测方法的阈值差异高达 4,但所有方法都能够检测到至少 20 CFU/5 μl(52 fg DNA/5 μl)的样本,其中 Diagenode 试剂盒的临床灵敏度最高。