一种用于检测少量肠道内容物中火鸡轮状病毒的改良基因组电泳分型程序。
A modified genome electropherotyping procedure for detecting turkey rotaviruses in small volumes of intestinal contents.
作者信息
Theil K W
机构信息
Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
出版信息
Avian Dis. 1987 Oct-Dec;31(4):899-903.
PMID:2831872
Abstract
A modified extraction procedure, suitable for the quick extraction of viral dsRNA from small quantities of intestinal contents, was developed. Of the 70 specimens positive for dsRNA viruses by genome electropherotyping with preparations derived by the standard extraction procedure, 56 (80%) were positive for virus with preparations derived by the modified procedure.
摘要
开发了一种改良的提取方法，适用于从少量肠道内容物中快速提取病毒双链RNA。在通过标准提取方法制备的样本经基因组电泳分析检测出双链RNA病毒呈阳性的70个样本中，56个（80%）经改良方法制备的样本病毒检测呈阳性。
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