Malpartida F, Zalacaín M, Jiménez A, Davies J
Biochem Biophys Res Commun. 1983 Nov 30;117(1):6-12. doi: 10.1016/0006-291x(83)91533-4.
The gene encoding the phosphotransferase enzyme that modifies hygromycin B in its producing organism Streptomyces hygroscopicus, has been cloned in the Streptomyces vector pIJ41. Two plasmids, pFM4 and pFM6, containing 2.1 and 19.6 kb inserts of Streptomyces hygroscopicus DNA, respectively, which express the modifying enzyme, have been isolated. A 3.1 kb PstI restriction fragment from pFM4 was inserted in the Streptomyces vector pIJ350 and the resulting plasmids, pMZ11.1 and pMZ11.2, express the hygromycin B-resistance phenotype. The utility of this dominant marker for cloning experiments is discussed in the text.
编码磷酸转移酶的基因可对其产生菌吸水链霉菌中的潮霉素B进行修饰，该基因已被克隆到链霉菌载体pIJ41中。已分离出两个质粒pFM4和pFM6，分别含有2.1 kb和19.6 kb吸水链霉菌DNA插入片段，它们可表达修饰酶。将来自pFM4的3.1 kb PstI限制性片段插入链霉菌载体pIJ350中，所得质粒pMZ11.1和pMZ11.2可表达潮霉素B抗性表型。文中讨论了这种显性标记在克隆实验中的实用性。
