UNESCO-IHE, Institute for Water Education, PO Box 3015, 2601 DA Delft, The Netherlands.
UNESCO-IHE, Institute for Water Education, PO Box 3015, 2601 DA Delft, The Netherlands.
Bioresour Technol. 2017 Sep;240:214-222. doi: 10.1016/j.biortech.2017.03.003. Epub 2017 Mar 6.
Microorganisms from an anaerobic methane oxidizing sediment were enriched with methane gas as the substrate in a biotrickling filter (BTF) using thiosulfate as electron acceptor for 213days. Thiosulfate disproportionation to sulfate and sulfide were the dominating sulfur conversion process in the BTF and the sulfide production rate was 0.5mmollday. A specific group of sulfate reducing bacteria (SRB), belonging to the Desulforsarcina/Desulfococcus group, was enriched in the BTF. The BTF biomass showed maximum sulfate reduction rate (0.38mmollday) with methane as sole electron donor, measured in the absence of thiosulfate in the BTF. Therefore, a BTF fed with thiosulfate as electron acceptor can be used to enrich SRB of the DSS group and activate the inoculum for anaerobic oxidation of methane coupled to sulfate reduction.
从厌氧甲烷氧化沉积物中分离出的微生物，在以硫代硫酸盐为电子受体的生物滴滤器（BTF）中，以甲烷气体为底物进行了 213 天的富集。硫代硫酸盐歧化为硫酸盐和硫化物是 BTF 中主要的硫转化过程，硫化物的生成速率为 0.5mmol/L·天。在 BTF 中富集了一种特定的硫酸盐还原菌（SRB），属于脱硫弧菌/脱硫球菌属。当 BTF 中不存在硫代硫酸盐时，BTF 生物量表现出最大的硫酸盐还原速率（0.38mmol/L·天），以甲烷作为唯一的电子供体进行测量。因此，以硫代硫酸盐作为电子受体的 BTF 可用于富集 DSS 组的 SRB，并激活接种物进行与硫酸盐还原偶联的厌氧甲烷氧化。
