Laboratory of Environmental Science and Engineering, School of Mining and Metallurgical Engineering, National Technical University of Athens (NTUA), 9 Heroon Polytechniou, 15780, Athens, Greece.
Department of Environmental Engineering and Water Technology, UNESCO-ΙΗΕ, Westvest 7, 2611-AX, Delft, The Netherlands.
Bioprocess Biosyst Eng. 2021 Apr;44(4):855-874. doi: 10.1007/s00449-020-02495-2. Epub 2021 Feb 10.
The process dynamics of anaerobic oxidation of methane (AOM) coupled to sulfate reduction (SR), and the potential role of elemental sulfur as intermediate are presented in this paper. Thermodynamic screening and experimental evidence from the literature conclude that a prominent model to describe AOM-SR is based on the concept that anaerobic methane oxidation proceeds through the production of the intermediate elemental sulfur. Two microbial groups are involved in the process: (a) anaerobic methanotrophs (ANME-2) and (b) Desulfosarcina/Desulfococcus sulfur reducers cluster (DSS). In this work, a dynamic model was developed to explore the interactions between biotic and abiotic processes to simulate the microbial activity, the chemical composition and speciation of the liquid phase, and the gas phase composition in the reactor headspace. The model includes the microbial kinetics for the symbiotic growth of ANME-2 and DSS, mass transfer phenomena between the gas and liquid phase for methane, hydrogen sulfide, and carbon dioxide and acid-base reactions for bicarbonate, sulfide, and ammonium. A data set from batch experiments, running for 250 days in artificial seawater inoculated with sediment from Marine Lake Grevelingen (The Netherlands) was used to calibrate the model. The inherent characteristics of AOM-SR make the identification of the kinetic parameters difficult due to the high correlation between them. However, by meaningfully selecting a set of kinetic parameters, the model simulates successfully the experimental data for sulfate reduction and sulfide production. The model can be considered as the basic structure for simulating continuous flow three-phase engineered systems based on AOM-SR.
本文介绍了甲烷厌氧氧化（AOM）与硫酸盐还原（SR）偶联的过程动力学，以及单质硫作为中间产物的潜在作用。热力学筛选和文献中的实验证据得出结论，一个描述 AOM-SR 的突出模型基于这样的概念，即厌氧甲烷氧化通过产生中间产物单质硫来进行。该过程涉及两个微生物群体：（a）厌氧甲烷氧化菌（ANME-2）和（b）脱硫弧菌/脱硫球菌还原菌簇（DSS）。在这项工作中，开发了一个动态模型来探索生物和非生物过程之间的相互作用，以模拟微生物活性、液相的化学组成和形态以及反应器气相组成。该模型包括 ANME-2 和 DSS 共生生长的微生物动力学、甲烷、硫化氢和二氧化碳在气液相间的传质现象以及碳酸氢盐、硫化物和铵的酸碱反应。使用从人工海水中进行的 250 天批处理实验获得的数据集，该数据集接种了来自荷兰 Grevelingen 海洋湖的沉积物，对模型进行了校准。由于它们之间的高度相关性，AOM-SR 的固有特性使得识别动力学参数变得困难。然而，通过有意义地选择一组动力学参数，该模型成功地模拟了硫酸盐还原和硫化物生成的实验数据。该模型可以被认为是基于 AOM-SR 模拟连续流动三相工程系统的基本结构。
