Myrmel H
Department of Microbiology and Immunology, Gade Institute, Haukeland Hospital, Bergen, Norway.
APMIS. 1988 Mar;96(3):280-1. doi: 10.1111/j.1699-0463.1988.tb05302.x.
Seventy serum samples from 54 patients with clinically suspected infectious mononucleosis were examined, using the Paul-Bunnell-Davidsohn test, a rapid slide test and an ELISA for the detection of IgM antibodies against the viral capsid antigen (VCA) of Epstein-Barr virus. The ELISA technique was the most sensitive, detecting IgM in 29 sera, whereas only 19 sera were positive in the Paul-Bunnell-Davidsohn test (titre greater than or equal to 64). However, using a titre of greater than or equal to 32 as a diagnostic level, the number of positive sera was 23, the same as for the rapid slide test. There was a high agreement (91%) between the heterophile antibodies tests and the VCA-IgM ELISA. The Paul-Bunnell-Davidsohn test still holds a place in the serologic diagnosis of infectious mononucleosis, but sera should always be tested for VCA-IgM in cases of heterophile antibodies negative mononucleosis.
对54例临床疑似传染性单核细胞增多症患者的70份血清样本进行了检测，采用保罗 - 邦内尔 - 戴维森试验、快速玻片试验和酶联免疫吸附测定法（ELISA）来检测针对EB病毒衣壳抗原（VCA）的IgM抗体。ELISA技术最为灵敏，在29份血清中检测到IgM，而保罗 - 邦内尔 - 戴维森试验中只有19份血清呈阳性（滴度大于或等于64）。然而，以大于或等于32的滴度作为诊断标准时，阳性血清数量为23份，与快速玻片试验相同。嗜异性抗体试验和VCA-IgM ELISA之间存在高度一致性（91%）。保罗 - 邦内尔 - 戴维森试验在传染性单核细胞增多症的血清学诊断中仍占有一席之地，但在嗜异性抗体阴性的单核细胞增多症病例中，血清应始终检测VCA-IgM。
