一种改良的爱泼斯坦-巴尔病毒特异性IgM抗体免疫荧光检测法。

A modified immunofluorescence test for Epstein-Barr virus-specific IgM antibody.

作者信息

Harnett G B, Palmer C A, Bucens M R

出版信息

J Virol Methods. 1985 Oct;12(1-2):25-30. doi: 10.1016/0166-0934(85)90004-7.

Abstract

The fluorescent antibody (FA) test for Epstein-Barr virus (EBV)-specific IgM antibody was improved by the use of sodium butyrate to induce a higher level of EBV antigen expression in P3HR-1 slide preparations and by removal of rheumatoid factor (RF) and IgG antibodies from test sera by means of adsorption with suspensions of Sepharose-IgG and Streptococcus pyogenes strain AR1. This method was compared with the Paul-Bunnell test (PB) on 1106 sera submitted to a routine virus diagnostic laboratory for infectious mononucleosis serology and 96.4% of sera showed concordant results. Thus the EBV-IgM-FA method was suitable for routine diagnostic use. However, it proved helpful to test EBV-IgM positive sera by PB to assist in the detection of cross-reacting IgM antibodies sometimes present.

摘要

通过使用丁酸钠提高P3HR-1载玻片制剂中EB病毒（EBV）抗原的表达水平，并通过用琼脂糖-免疫球蛋白G（Sepharose-IgG）悬液和化脓性链球菌AR1菌株吸附来去除检测血清中的类风湿因子（RF）和免疫球蛋白G（IgG）抗体，改进了用于检测EBV特异性IgM抗体的荧光抗体（FA）试验。将该方法与保罗-邦内尔试验（PB）对1106份提交给常规病毒诊断实验室进行传染性单核细胞增多症血清学检测的血清进行了比较，96.4%的血清显示结果一致。因此，EBV-IgM-FA方法适用于常规诊断。然而，事实证明，用PB检测EBV-IgM阳性血清有助于检测有时存在的交叉反应性IgM抗体。