Kitchener H C
Department of Midwifery, University of Glasgow, Queen Mother's Hospital, Yorkhill.
Br J Obstet Gynaecol. 1988 Feb;95(2):182-91. doi: 10.1111/j.1471-0528.1988.tb06849.x.
The reported association between genital infection by herpes simplex virus (HSV) type 2 and cervical neoplasia has been investigated at a molecular level. Tissue culture of over 50 cervical cancers resulted in the development of several cell lines but none of these expressed HSV information. The permanent cell line, Cx106, which was generated was found to contain multiple genome copies of human papillomavirus (HPV) type 16. Southern blot analysis of nine cervical cancers showed an authentic 2.8 Kb fragment of HSV-2 DNA in one of the tumours. This piece of viral DNA maps in a morphological transforming region of the HSV genome. Superinfection of cervical cancers with temperature-sensitive (ts) mutants of HSV 1 and 2 did not generate recombinant virus, suggesting tht HSV DNA was not present in the tumours studied. An attempt to identify latent HSV close to the cervix in organ cultures of uterosacral ligament tissues resulted in reactivation of virus in several instances. These results are discussed against a background of decline in acceptance by many, for the HSV link with cervical cancer, and the dramatic impact of the discovery of a close relation between HPV type 16 and lower genital tract neoplasia.
已在分子水平上对报告的2型单纯疱疹病毒（HSV）生殖器感染与宫颈癌之间的关联进行了研究。对50多种宫颈癌进行组织培养，产生了几种细胞系，但这些细胞系均未表达HSV信息。所产生的永久性细胞系Cx106被发现含有16型人乳头瘤病毒（HPV）的多个基因组拷贝。对9例宫颈癌进行的Southern印迹分析显示，其中1例肿瘤中有一条真实的2.8kb HSV-2 DNA片段。这段病毒DNA定位于HSV基因组的一个形态转化区域。用HSV 1和2的温度敏感（ts）突变体对宫颈癌进行超感染未产生重组病毒，这表明在所研究的肿瘤中不存在HSV DNA。在子宫骶韧带组织的器官培养中，试图在宫颈附近鉴定潜伏性HSV，结果在若干情况下导致病毒重新激活。在许多人对HSV与宫颈癌之间联系的认可度下降以及发现16型HPV与下生殖道肿瘤之间密切关系所产生的巨大影响的背景下，对这些结果进行了讨论。
