Kartal H, Comu F M, Kucuk A, Polat Y, Dursun A D, Arslan M
Bratisl Lek Listy. 2017;118(3):133-136. doi: 10.4149/BLL_2017_026.
Erythrocyte deformability and plasma viscosity are of crucial importance for the perfusion of tissues and organs. The aim of this study was to evaluate the effect of apelin-13 on erythrocyte deformability during IR heart injury in diabetic rats.
Eighteen Wistar Albino rats were included in the study after streptozotocin (55 mg/kg) treatment for four weeks of observation for diabetes existence. The animals were randomly assigned to one of five experimental groups. In the Group C, DC (sham-control group) and DCA (sham-control group-apelin-13), the coronary artery was not occluded or re-perfused. In the Group DIR, a branch of the left coronary artery was occluded for 30 minutes followed by 90 minutes of re-perfusion to produce IR. In the Group DIRA, a branch of the left coronary artery was occluded for 30 minutes followed by 90 minutes of re-perfusion to produce IR, and apelin-13 was administrated via 10 µg.kg-1 IP route 30 minutes before ligating the left coronary artery.Deformability measurements were performed in erythrocyte suspensions containing Htc 5% in a PBS buffer.
The deformability index was significantly increased in diabetic rats; however, it was similar in Group DC, DCA and DIRA. It was significantly increased in the Group DIR when compared to the Group C, DIRA, DCA and DC. The relative resistance was increased in IR models.
Erythrocyte deformability was decreased in rats having diabetes and IR injury. This injury might lead to further problems in microcirculation. It was shown that apeline-13 may be useful in enhancing the adverse effects of this type of injury (Fig. 1, Ref. 35).
红细胞变形性和血浆黏度对组织和器官的灌注至关重要。本研究旨在评估apelin-13对糖尿病大鼠心脏缺血再灌注损伤期间红细胞变形性的影响。
18只Wistar白化大鼠经链脲佐菌素（55mg/kg）治疗4周后纳入研究，观察糖尿病情况。动物被随机分为五个实验组之一。在C组、DC（假手术对照组）和DCA（假手术对照组-apelin-13）中，冠状动脉未闭塞或再灌注。在DIR组中，左冠状动脉的一个分支闭塞30分钟，然后再灌注90分钟以产生缺血再灌注。在DIRA组中，左冠状动脉的一个分支闭塞30分钟，然后再灌注90分钟以产生缺血再灌注，并在结扎左冠状动脉前30分钟通过腹腔注射10μg·kg-1的途径给予apelin-13。在含有5%血细胞比容的PBS缓冲液的红细胞悬液中进行变形性测量。
糖尿病大鼠的变形性指数显著增加；然而，DC组、DCA组和DIRA组相似。与C组、DIRA组、DCA组和DC组相比，DIR组显著增加。缺血再灌注模型中的相对阻力增加。
糖尿病和缺血再灌注损伤大鼠的红细胞变形性降低。这种损伤可能导致微循环进一步出现问题。结果表明，apelin-13可能有助于增强这类损伤的不良反应（图1，参考文献35）。
