Tazehkand M Norizadeh, Hajipour O, Moridikia F, Moridikia A, Valipour E, Yilmaz M B, Topaktas M
Bratisl Lek Listy. 2017;118(3):149-152. doi: 10.4149/BLL_2017_029.
4-methylimidazole is widely used in pharmaceuticals, photographic and agricultural chemicals. The substance is extensively found in many human and animals foods. In this research, anticancer effect of the 4-MEI was studded using MTT test using MCF-7 cell line. Effect of the 4-MEI on apoptosis or necrosis was analyzed by DNA fragmentation assay using Swiss Albino rats as a model organism. Antioxidant effect of the substance was investigated by assaying protective effect of the substance on circular plasmid DNA against H2O2 as an oxidative agent. 4-MEI showed inhibitory effect on proliferation of MCF-7 cell line by all concentrations and the decrement was significant and concentration dependent. Result of DNA fragmentation assay showed 4-MEI concentrations dependent of smear formation showing necrotic effects of the 4-MEI on mouse cells. Also, the 4-MEI showed a good antioxidant activity and protective effect against H2O2.
The result of this study showed that 4MEI has significant antioxidant and anti-cancer effect. Also, according to the result, 4-MEI has necrotic effects on mouse cells (Fig. 3, Ref. 21).
4-甲基咪唑广泛应用于制药、摄影和农用化学品中。该物质在许多人类和动物食品中广泛存在。在本研究中，使用MCF-7细胞系通过MTT试验研究了4-MEI的抗癌作用。以瑞士白化大鼠为模型生物，通过DNA片段化分析来分析4-MEI对凋亡或坏死的影响。通过检测该物质对环状质粒DNA免受作为氧化剂的H2O2的保护作用来研究该物质的抗氧化作用。4-MEI在所有浓度下均对MCF-7细胞系的增殖表现出抑制作用，且抑制作用显著且呈浓度依赖性。DNA片段化分析结果显示，4-MEI浓度依赖性地形成涂片，表明4-MEI对小鼠细胞具有坏死作用。此外，4-MEI表现出良好的抗氧化活性和对H2O2的保护作用。
本研究结果表明，4-MEI具有显著的抗氧化和抗癌作用。此外，根据结果，4-MEI对小鼠细胞具有坏死作用（图3，参考文献21）。
