Norizadeh Tazehkand M
Bratisl Lek Listy. 2017;118(5):265-268. doi: 10.4149/BLL_2017_052.
4-MEI (4-Methylimidazole) is used as a chemical intermediate, crude material or component in the manufacture of pharmaceuticals, photographic and photothermographic chemicals, dyes and pigments and agricultural chemicals. 4-MEI is unintentionally found in our food. Caramel colour (which is the most used beverage colouring and food), dark beers and common brands of cola drinks may comprise more than 100 μg of this compound per 12-ounce serving. 4-MEI is widely used by people and colon cancer is common in our countries. So, it was decided to do in vitro analysis of anti-cancer effect of 4-MEI by MTT test using htc-116 cell line.In this study, mouse Htc-116 cell line was treated with 4-MEI concentrations of 300, 450, 600 and 750 µg/mL for 24 hours and 48 hours periods, after that antiproliferative effect of the 4-MEI was studied by MTT assay. In this study 4-MEI at highest concentration of 24h and at all concentration for 48 h treatment time significantly inhibited cell proliferation when it was compared to control. Also, exposing to the 4-MEI for 48 hours led to a decrease in cells proliferation by concentration dependent manner. This result showed that 4-MEI had anticancer effect in htc-116 cells. However, it has to be evaluated with different new studies (Tab. 1, Fig. 4, Ref. 19).
4-甲基咪唑（4-MEI）在制药、照相和光热成像化学品、染料和颜料以及农用化学品的制造中用作化学中间体、原材料或成分。4-甲基咪唑在我们的食物中是意外发现的。焦糖色素（这是最常用的饮料色素和食品色素）、黑啤酒和常见品牌的可乐饮料每12盎司的含量可能超过100微克这种化合物。4-甲基咪唑被人们广泛使用，并且在我们国家结肠癌很常见。因此，决定使用htc-116细胞系通过MTT试验对4-甲基咪唑的抗癌作用进行体外分析。在本研究中，将小鼠Htc-116细胞系用浓度为300、450、600和750微克/毫升的4-甲基咪唑处理24小时和48小时，之后通过MTT试验研究4-甲基咪唑的抗增殖作用。在本研究中，与对照组相比，4-甲基咪唑在24小时最高浓度以及48小时所有浓度处理时均显著抑制细胞增殖。此外，将细胞暴露于4-甲基咪唑48小时导致细胞增殖以浓度依赖性方式下降。该结果表明4-甲基咪唑在htc-116细胞中具有抗癌作用。然而，必须通过不同的新研究进行评估（表1、图4、参考文献19）。
