砷酸氢盐在猪粪消化过程中对抗生素抗性基因和微生物群落的作用机制及影响。
Mechanisms and effects of arsanilic acid on antibiotic resistance genes and microbial communities during pig manure digestion.
机构信息
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Research Center of Recycle Agricultural Engineering and Technology of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi 712100, China.
出版信息
Bioresour Technol. 2017 Jun;234:217-223. doi: 10.1016/j.biortech.2017.03.025. Epub 2017 Mar 8.
High concentrations of residual arsanilic acid occur in pig manure due to its use in feed to promote growth and control diseases. This study compared the effects of arsanilic acid at three concentrations (0, 325, and 650mg/kg dry pig manure) on the abundance of antibiotic resistance genes (ARGs) and the microbial community during anaerobic digestion. Addition of 650mg/kg arsanilic acid enhanced the absolute abundances of tetC, sul2, ermB, and gyrA more than twofold in the digestion product. Redundancy analysis indicated that the change in the microbial community structure was the main driver of variation in the ARGs profile. The As resistance gene arsC co-occurred with four ARGs and intI1, possibly causing the increase in ARGs under pressure by arsanilic acid. High arsanilic acid concentrations can increase the risk of ARGs occurring in anaerobic digestion products. The amount of arsanilic acid used as a feed additive should be controlled.
高浓度的残留阿散酸由于其在饲料中的使用而存在于猪粪中，以促进生长和控制疾病。本研究比较了阿散酸在三个浓度（0、325 和 650mg/kg 干猪粪）下对厌氧消化过程中抗生素抗性基因（ARGs）丰度和微生物群落的影响。添加 650mg/kg 的阿散酸使消化产物中 tetC、sul2、ermB 和 gyrA 的绝对丰度增加了两倍以上。冗余分析表明，微生物群落结构的变化是 ARGs 谱变异的主要驱动因素。砷抗性基因 arsC 与四个 ARGs 和 intI1 共同出现，可能导致阿散酸压力下 ARGs 的增加。高浓度的阿散酸会增加 ARGs 出现在厌氧消化产物中的风险。作为饲料添加剂使用的阿散酸的量应该得到控制。
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