College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, China; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria 3010, Australia; Huayu Agricultural Science and Technology Co., LTD, Handan 057153, China.
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria 3010, Australia.
Bioresour Technol. 2020 May;303:122906. doi: 10.1016/j.biortech.2020.122906. Epub 2020 Jan 27.
Lignite could be used to promote methane production during high-solid anaerobic co-digestion (HS-AcoD) of pig manure, however, the effects of lignite amendment on the fate of ARGs during HS-AcoD are unknown. Here, we explored the influence of lignite (0%, 8%, 16%, 32%, and 64%) on the fate of ARGs during HS-AcoD of pig manure. The results showed that 16% lignite reduced the absolute abundance of ARGs by 28.71% compared with the 0% lignite treatment. Variation partitioning analysis suggested the combined effect of microbial community, mobile genetic elements (MGEs) and environmental factors was the major driver shaping the pattern of ARGs. The potential hosts of ARGs were Bifidobacterium, Lactobacillus, Tissierella and Streptococcus. Structural equation models analysis suggested lignite indirectly impacted the pattern of ARGs by significantly reducing the abundance of microbial community and MGEs. These findings give an insight into the mechanistic understanding of the lignite influence on the reduction of ARGs during HS-AcoD.
褐煤可以用于促进高固厌氧共消化（HS-AcoD）过程中甲烷的产生，然而，褐煤添加对 HS-AcoD 过程中抗生素抗性基因（ARGs）命运的影响尚不清楚。在这里，我们研究了褐煤（0%、8%、16%、32%和 64%）对猪粪 HS-AcoD 过程中 ARGs 命运的影响。结果表明，与 0%褐煤处理相比，16%褐煤使 ARGs 的绝对丰度降低了 28.71%。变异分解分析表明，微生物群落、移动遗传元件（MGEs）和环境因素的综合效应是塑造 ARGs 模式的主要驱动因素。ARGs 的潜在宿主是双歧杆菌、乳杆菌、栖瘤胃球菌和链球菌。结构方程模型分析表明，褐煤通过显著降低微生物群落和 MGEs 的丰度，间接地影响了 ARGs 的模式。这些发现深入了解了褐煤对 HS-AcoD 过程中 ARGs 减少的影响的机制理解。
