Shemer Benjamin, Koshet Ori, Yagur-Kroll Sharon, Belkin Shimshon
Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Curr Opin Biotechnol. 2017 Jun;45:113-119. doi: 10.1016/j.copbio.2017.03.003. Epub 2017 Mar 17.
Since its introduction as an explosive in the late 19th century, 2,4,6-trinitrotoluene (TNT), along with other explosive compounds, has left numerous environmental marks. One of these is widespread soil and water pollution by trace explosives in military proving grounds, manufacturing facilities, or actual battlefields. Another dramatic impact is that exerted by the millions of landmines and other explosive devices buried in large parts of the world, causing extensive loss of life, injuries, and economical damage. In this review we highlight recent advances in the design and construction of microbial bioreporters, molecularly engineered to generate a quantifiable dose-dependent signal in the presence of trace amounts of explosives. Such sensor strains may be employed for monitoring environmental pollution as well as for the remote detection of buried landmines.
自19世纪末作为炸药被引入以来，2,4,6-三硝基甲苯（TNT）与其他炸药化合物一起留下了众多环境印记。其中之一是军事试验场、制造设施或实际战场中微量炸药对土壤和水的广泛污染。另一个巨大影响是埋在世界大部分地区的数百万枚地雷和其他爆炸装置所造成的，导致大量人员伤亡和经济损失。在本综述中，我们重点介绍了微生物生物报告器设计和构建方面的最新进展，这些生物报告器经过分子工程改造，能够在痕量炸药存在的情况下产生可量化的剂量依赖性信号。此类传感菌株可用于监测环境污染以及远程探测埋藏的地雷。
