单纯疱疹病毒 1 型 ICP27 通过调节 Daxx 泛素化来抑制 NF-κB 活性。
HSV-1 ICP27 represses NF-κB activity by regulating Daxx sumoylation.
机构信息
Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
出版信息
BMB Rep. 2017 May;50(5):275-280. doi: 10.5483/bmbrep.2017.50.5.010.
Herpes simplex virus type 1 ICP27 is a multifunctional protein responsible for viral replication, late gene expression, and reactivation from latency. ICP27 interacts with various cellular proteins, including Daxx. However, the role of interaction between ICP27 and Daxx is largely unknown. Since Daxx is known to repress NF-κB activity, there is a possibility that ICP27 may influence the inhibitory effect of Daxx on NF-κB activity. In this study, we tested whether ICP27 affects the NF-κB activity through its interaction with Daxx. Interestingly, ICP27 enhanced the Daxx-mediated repression of NF-κB activity. In addition, we found that sumoylation of Daxx regulates its interaction with p65. ICP27 binds to Daxx, inhibits Daxx sumoylation, and enhances p65 deacetylation induced by Daxx. Consequently, ICP27 represses the NF-B activity, by elevating the inhibitory effect of Daxx on NF-κB activity through desumoylation of Daxx. [BMB Reports 2017; 50(5): 275-280].
单纯疱疹病毒 1 型 ICP27 是一种多功能蛋白，负责病毒复制、晚期基因表达和潜伏后激活。ICP27 与各种细胞蛋白相互作用，包括 Daxx。然而，ICP27 与 Daxx 之间相互作用的作用在很大程度上尚不清楚。由于已知 Daxx 抑制 NF-κB 活性，因此 ICP27 可能会影响 Daxx 对 NF-κB 活性的抑制作用。在这项研究中，我们测试了 ICP27 是否通过与 Daxx 的相互作用影响 NF-κB 活性。有趣的是，ICP27 增强了 Daxx 介导的 NF-κB 活性抑制。此外，我们发现 Daxx 的 SUMO 化调节其与 p65 的相互作用。ICP27 结合 Daxx，抑制 Daxx SUMO 化，并增强 Daxx 诱导的 p65 去乙酰化。因此，ICP27 通过 Daxx 的去 SUMO 化，抑制 Daxx 对 NF-κB 活性的抑制作用，从而抑制 NF-κB 活性。[BMB 报告 2017；50(5): 275-280]。
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