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口服维生素 B12 在组织中的分布受其状态和形式的影响：大鼠实验研究。

Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats.

机构信息

Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus N, Denmark.

Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.

出版信息

Eur J Nutr. 2018 Jun;57(4):1459-1469. doi: 10.1007/s00394-017-1424-0. Epub 2017 Mar 20.

DOI:10.1007/s00394-017-1424-0
PMID:28321545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5960002/
Abstract

PURPOSE

Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[Co]Cbl and CN[Co]Cbl in Cbl-deficient and normal rats.

METHODS

Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[Co]Cbl (free or bound to bovine transcobalamin) and free CN[Co]Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [Co]Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods.

RESULTS

Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p < 0.0001). Cbl depletion increased endogenous Cbl ratios (tissue/plasma = k /k ) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl > CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma).

CONCLUSIONS

The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma.

摘要

目的

羟钴胺素（HOCbl）是食物中主要的钴胺素形式，而氰钴胺素（CNCbl）则常见于维生素丸和口服补充剂中。本研究比较了 Cbl 缺乏和正常大鼠单次口服 HO[Co]Cbl 和 CN[Co]Cbl 的吸收和分布。

方法

雄性 Wistar 大鼠（7 周龄）用含（n=15）或不含（n=15）Cbl 的 14 天饮食喂养。我们比较了胃管给予的 HO[Co]Cbl（与牛转钴胺素结合或不结合）和游离 CN[Co]Cbl 的摄取（每组饮食各 5 只）。大鼠在 24 小时后处死。收集血液、肝脏、肾脏、大脑、心脏、脾脏、肠、骨骼肌、24 小时尿液和粪便，并测量[Co]Cbl 含量。用常规方法分析组织和血浆中的内源性 Cbl。

结果

缺乏组大鼠的平均内源性血浆 Cbl 比正常组低 7 倍（190 vs. 1330 pmol/L，p<0.0001）。Cbl 缺乏增加了所有器官（除肾脏外）的内源性 Cbl 比值（组织/血浆=k/k），而肾脏的比值则显著降低。24 小时标记 Cbl 的积累表明 HOCbl>CNCbl（肝脏）和 CNCbl>HOCbl（大脑、肌肉和血浆）。

结论

大鼠的 Cbl 状态和给予的 Cbl 形式影响 24 小时内 Cbl 在组织和血浆中的积累。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/e76d5422acdd/394_2017_1424_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/54321ed2473e/394_2017_1424_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/2728dd0a1cdf/394_2017_1424_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/5ef44e075529/394_2017_1424_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/933882034182/394_2017_1424_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/801471f3c7fe/394_2017_1424_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/e76d5422acdd/394_2017_1424_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/54321ed2473e/394_2017_1424_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/2728dd0a1cdf/394_2017_1424_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/5ef44e075529/394_2017_1424_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/933882034182/394_2017_1424_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/801471f3c7fe/394_2017_1424_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736f/5960002/e76d5422acdd/394_2017_1424_Fig6_HTML.jpg

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