Department of Pathology, Deenanath Mangeshkar Hospital and Research Center, Pune 411004, India.
Department of Clinical Biochemistry and Institute of Clinical Medicine, Aarhus University Hospital, DK-8200 Aarhus N, Denmark.
Nutrients. 2019 Feb 1;11(2):304. doi: 10.3390/nu11020304.
We assessed improvements in the vitamin B12 status of Indian lactovegetarians receiving four weeks supplementation with natural B12 in milk versus cyano-B12 in capsules. Three groups ( = 22, 23, 22) received daily oral doses of cyano-B12 (2 × 0.76 µg) or milk (2 × 200 mL) from a cow or buffalo (amounting to B12 ≈ 2 × 0.76 µg). Their blood was examined at baseline and each following week. The baselines (median (min/max)) indicated a low B12 status: plasma B12 (116(51/314)) pmol/L, holotranscobalamin (holoTC) (30(7/119)) pmol/L, total homocysteine (Hcy) (24(10/118)) µmol/L, methylmalonic acid (MMA) (0.58(0.15/2.2)) µmol/L and combined B12 index (cB12) (-1.32 - (-3.12/+0.29)). Shifts from the baselines (B12, holoTC, cB12) and ratios to the baselines (Hcy, MMA) were analyzed over time. The cyano-B12 treatment gave more total B12 in plasma at week one (+29 pmol/L, = 0.004) but showed no further increase. Other biomarkers changed more comparably between the three groups ( ≥ 0.05): holoTC showed a transient spike that leveled off, Hcy finally decreased to 0.8 × baseline, while MMA showed marginal changes. The combined indexes improved comparably ( = 0.6) in all groups (+0.2(-0.3/+0.9), ≤ 0.002). In conclusion, the tested formulations similarly improved B12 status, but did not normalize it.
我们评估了印度严格素食者在四周内分别接受牛奶中天然 B12 与胶囊中氰钴胺（cyano-B12）补充后维生素 B12 状况的改善情况。三组（n = 22、23、22）分别接受每日口服剂量的氰钴胺（2 × 0.76 µg）或来自奶牛或水牛的牛奶（2 × 200 mL，B12 量约为 2 × 0.76 µg）。他们的血液在基线和之后每周进行检查。基线（中位数（最小/最大））显示出低 B12 状态：血浆 B12（116（51/314））pmol/L，全钴胺素（holoTC）（30（7/119））pmol/L，总同型半胱氨酸（Hcy）（24（10/118））µmol/L，甲基丙二酸（MMA）（0.58（0.15/2.2））µmol/L 和综合 B12 指数（cB12）（-1.32-（-3.12/+0.29））。分析了从基线（B12、holoTC、cB12）的变化和与基线的比值（Hcy、MMA）随时间的变化。氰钴胺治疗在第 1 周使血浆中的总 B12 增加了 29 pmol/L（P = 0.004），但没有进一步增加。其他生物标志物在三组之间变化更为相似（≥0.05）：holoTC 表现出短暂的尖峰，随后稳定下来，Hcy 最终降至 0.8×基线，而 MMA 则略有变化。所有组的综合指数均得到了相似的改善（P = 0.6）（+0.2（-0.3/+0.9），P ≤ 0.002）。总之，测试的配方同样改善了 B12 状况，但没有使其正常化。
