Maľucká Lucia Ungvarská, Harvanova Jarmila, Uhrinová Anna, Salayová Aneta, Pavlík Martin, Rajtar Martin
Department of Chemistry, Biochemistry, and Biophysics, Institute of Pharmaceutical Chemistry, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic.
Faculty of Forestry, Department of Integrated Forest and Landscape Protection, Technical University in Zvolen, Zvolen, Slovak Republic.
Int J Med Mushrooms. 2018;20(6):595-605. doi: 10.1615/IntJMedMushrooms.2018026548.
This research studies the influence of substrate on the antioxidant activity of alcohol extracts of Paecilomyces hepiali. We used corn, rice, millet, and peas as substrates. Antioxidant activity was measured with the DPPH radical scavenging method. Concentrations of extracts (6.25, 3.12, 1.56, 0.78, and 0.39 mg/mL) were applied in all evaluations. Overall antioxidant activity was expressed as the concentration of substrate that decreased DPPH radical levels by 50% (IC50DPPH) for 7 methanol and 7 ethanol extracts. A comparison of IC50DPPH allowed us to conclude that the methanol extracts are more active in scavenging stable DPPH radicals than are the ethanol extracts. The substrate with antioxidant properties most suitable for cultivation of P. hepiali was rice supplemented with non-defatted soy flour. The extract most effective in scavenging stable radicals was the methanol extract of sample 4 (IC50DPPH = 2.33 mg/mL) cultivated on rice with nondefatted soy flour. The methanol extract of sample 7 cultivated on peas was less effective (IC50DPPH = 11.50 mg/mL). By crystallizing these extracts, we managed to obtain sufficient quantities of 6 samples in a solid state, for which infrared spectra were measured and confirmed the presence of amino acids in the extracts.
本研究探讨了培养基质对蝙蝠蛾拟青霉乙醇提取物抗氧化活性的影响。我们使用玉米、大米、小米和豌豆作为培养基质。采用DPPH自由基清除法测定抗氧化活性。在所有评估中均应用了不同浓度的提取物（6.25、3.12、1.56、0.78和0.39 mg/mL）。总体抗氧化活性以使7种甲醇提取物和7种乙醇提取物的DPPH自由基水平降低50%的培养基质浓度（IC50DPPH）表示。通过比较IC50DPPH，我们得出结论，甲醇提取物在清除稳定的DPPH自由基方面比乙醇提取物更具活性。最适合蝙蝠蛾拟青霉培养的具有抗氧化特性的培养基质是添加了未脱脂大豆粉的大米。在添加了未脱脂大豆粉的大米上培养的样品4的甲醇提取物在清除稳定自由基方面最有效（IC50DPPH = 2.33 mg/mL）。在豌豆上培养的样品7的甲醇提取物效果较差（IC50DPPH = 11.50 mg/mL）。通过对这些提取物进行结晶，我们成功获得了足够数量的6种固态样品，并对其进行了红外光谱测量，证实提取物中存在氨基酸。
