Das Aparajita Roy, Borthakur Madhusmita, Saha Ajay Krishna, Joshi Santa Ram, Das Panna
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, India.
Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya, India.
Int J Med Mushrooms. 2017;19(1):55-63. doi: 10.1615/IntJMedMushrooms.v19.i1.60.
The aim of this study was to characterize 3 wild culinary-medicinal mushrooms using molecular tools and to analyze their antioxidant activity. Antioxidant properties were studied by evaluating free radical scavenging, reducing power, and chelating effect. The mushrooms were identified as Lentinus squarrosulus, L. tuber-regium, and Macrocybe gigantean by amplifying internal transcribed spacer regions of ribosomal DNA. The results demonstrated that the methanolic extract of M. gigantean has the highest free radical scavenging effect and chelating effect, whereas the methanolic extract of L. squarrosulus has the highest reducing power. The highest total phenol content and the most ascorbic acid were found in the M. gigantean extracts. Among the 3 mushroom extracts, M. gigantean displayed the most potent antioxidant activity. Molecular characterization using the nuclear ribosomal internal transcribed spacer region as a universal DNA marker was an effective tool in the identification and phylogenetic analysis of the studied mushrooms. The study also indicated that these wild macrofungi are rich sources of natural antioxidants.
本研究的目的是使用分子工具对3种野生食药用蘑菇进行特征描述，并分析它们的抗氧化活性。通过评估自由基清除能力、还原能力和螯合效应来研究抗氧化特性。通过扩增核糖体DNA的内部转录间隔区，将这些蘑菇鉴定为刺芹侧耳、雷丸和高大环柄菇。结果表明，高大环柄菇的甲醇提取物具有最高的自由基清除效果和螯合效应，而刺芹侧耳的甲醇提取物具有最高的还原能力。高大环柄菇提取物中的总酚含量最高，抗坏血酸含量也最多。在这3种蘑菇提取物中，高大环柄菇表现出最强大的抗氧化活性。使用核糖体DNA内部转录间隔区作为通用DNA标记进行分子特征描述，是所研究蘑菇鉴定和系统发育分析的有效工具。该研究还表明，这些野生大型真菌是天然抗氧化剂的丰富来源。
