State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao.
Carbohydr Polym. 2013 Sep 12;97(2):398-405. doi: 10.1016/j.carbpol.2013.04.101. Epub 2013 May 9.
Polysaccharides from Ganoderma spp. and their adulterants were firstly investigated and compared using saccharide mapping, enzymatic (endo-1,3-β-D-glucanase and pectinase) digestion followed by polysaccharide analysis using carbohydrate gel electrophoresis analysis. The results showed that both 1,3-β-D-glucosidic and 1,4-α-D-galactosiduronic linkages were existed in Lingzhi (Ganoderma lucidum and Ganoderma sinense), and the similarity of polysaccharides from G. lucidum and G. sinense was high, which may contribute to rational use of Lingzhi. Different species of Ganoderma and their adulterants can be differentiated based on the saccharide mapping, which is helpful to well understand the structural characters of polysaccharides from different species of Ganoderma and to improve the quality control of polysaccharides in Lingzhi.
采用糖谱分析、酶解（内切 1,3-β-D-葡聚糖酶和果胶酶）后再用糖凝胶电泳分析对灵芝（赤芝和密纹薄芝）及其掺杂物中的多糖进行了首次研究和比较。结果表明，灵芝中的 1,3-β-D-葡糖苷键和 1,4-α-D-半乳糖醛酸键均存在，赤芝和密纹薄芝中的多糖相似度高，这可能有助于灵芝的合理使用。不同种灵芝及其掺杂物可根据糖谱分析进行区分，这有助于更好地了解不同种灵芝多糖的结构特征，并提高灵芝多糖的质量控制。
