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聚γ-谷氨酸/壳聚糖纳米凝胶极大地增强了 H1N1 大流行性流感疫苗的效力和异源交叉反应性。

Poly-γ-glutamic acid/chitosan nanogel greatly enhances the efficacy and heterosubtypic cross-reactivity of H1N1 pandemic influenza vaccine.

机构信息

Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.

Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.

出版信息

Sci Rep. 2017 Mar 21;7:44839. doi: 10.1038/srep44839.

DOI:10.1038/srep44839
PMID:28322289
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5359587/
Abstract

In 2009, the global outbreak of an influenza pandemic emphasized the need for an effective vaccine adjuvant. In this study, we examined the efficacy of poly-γ-glutamic acid/chitosan (PC) nanogel as an adjuvant for the influenza vaccine. PC nanogel significantly enhanced antigen-specific cross-presentation and cytotoxic T lymphocyte (CTL) activity. Compared with alum, the protective efficacy of the pandemic H1N1 influenza (pH1N1) vaccine was substantially increased by PC nanogel, with increased hemagglutination-inhibition titers, CTL activity, and earlier virus clearance after homologous and heterosubtypic [A/Philippines/2/82 (H3N2)] virus challenges. However, CD8 T cell-depleted mice displayed no protection against the heterosubtypic virus challenge after immunization with PC nanogel-adjuvanted pH1N1 vaccine. We also observed that using PC nanogel as a vaccine adjuvant had a dose-sparing effect and significantly enhanced the long-lasting protection of the pH1N1 vaccine. Together, these results suggest that PC nanogel is a promising vaccine adjuvant that could broadly prevent influenza virus infection.

摘要

2009 年，全球流感大流行凸显了有效疫苗佐剂的必要性。在这项研究中，我们研究了聚γ-谷氨酸/壳聚糖（PC）纳米凝胶作为流感疫苗佐剂的功效。PC 纳米凝胶显著增强了抗原特异性交叉呈递和细胞毒性 T 淋巴细胞（CTL）活性。与铝佐剂相比，PC 纳米凝胶显著提高了大流行性 H1N1 流感（pH1N1）疫苗的保护效力，增加了血凝抑制滴度、CTL 活性，并在同源和异源[A/菲律宾/2/82（H3N2）]病毒挑战后更早地清除病毒。然而，用 PC 纳米凝胶-adjuvanted pH1N1 疫苗免疫的 CD8 T 细胞耗竭小鼠在异源病毒挑战后没有得到保护。我们还观察到，使用 PC 纳米凝胶作为疫苗佐剂具有剂量节省效应，并显著增强了 pH1N1 疫苗的持久保护作用。综上所述，这些结果表明 PC 纳米凝胶是一种有前途的疫苗佐剂，可以广泛预防流感病毒感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/ca33bfe13e9f/srep44839-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/5548474c81c0/srep44839-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/7c02d9692949/srep44839-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/df0cfe8a307e/srep44839-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/0ab6a73eaec6/srep44839-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/a44d1516d001/srep44839-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/ca33bfe13e9f/srep44839-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/5548474c81c0/srep44839-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/7c02d9692949/srep44839-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/df0cfe8a307e/srep44839-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/0ab6a73eaec6/srep44839-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/a44d1516d001/srep44839-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d346/5359587/ca33bfe13e9f/srep44839-f6.jpg

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