Caro M, Sansone A, Amezaga J, Navarro V, Ferreri C, Tueros I
New Foods, AZTI, Astondo Bidea 609, 48160 Derio, Spain.
Food Funct. 2017 Apr 19;8(4):1652-1659. doi: 10.1039/c6fo01754a.
This study investigates the ability of a polyphenolic extract obtained from a wine lees by-product to modulate zebrafish lipid metabolism. Lees from a Spanish winery were collected and the polyphenolic extract was chemically characterised in terms of antioxidant capacity, total phenolic content and the individual main phenolic compounds. The effects of the extract on lipid metabolism were evaluated using a zebrafish animal model. Lees are rich in polyphenols (42.33 mg gallic acid equivalent per g dry matter) with high antioxidant capacity (56.04 mg Trolox equivalent per g dry matter), rutin and quercetin being their main identified polyphenols. The biological effects of lees extract included (i) a reduction in zebrafish embryos' fat reserve (40%), (ii) changes in the expression of lipid metabolism key genes, (iii) remodelling of the fatty acid content in phospholipid and triglyceride fractions of zebrafish embryos and (iv) reduction in the trans fatty acid content. On the whole, wine lees polyphenolic extract was effective at modulating zebrafish lipid metabolism evidencing remodelling effects and antioxidant properties that can be further developed for food innovation.
本研究调查了从葡萄酒酒渣副产品中获得的多酚提取物调节斑马鱼脂质代谢的能力。收集了一家西班牙酿酒厂的酒渣，并对多酚提取物的抗氧化能力、总酚含量和主要酚类化合物进行了化学表征。使用斑马鱼动物模型评估了该提取物对脂质代谢的影响。酒渣富含多酚（每克干物质含42.33毫克没食子酸当量），具有较高的抗氧化能力（每克干物质含56.04毫克Trolox当量），芦丁和槲皮素是其主要鉴定出的多酚。酒渣提取物的生物学效应包括：（i）斑马鱼胚胎脂肪储备减少（40%）；（ii）脂质代谢关键基因表达的变化；（iii）斑马鱼胚胎磷脂和甘油三酯部分脂肪酸含量的重塑；（iv）反式脂肪酸含量的降低。总体而言，葡萄酒酒渣多酚提取物在调节斑马鱼脂质代谢方面有效，证明了其重塑效应和抗氧化特性，可进一步用于食品创新。
