Park Y M, Stauffer G V
Department of Microbiology, University of Iowa, Iowa City 52242.
Gene. 1987;60(2-3):291-7. doi: 10.1016/0378-1119(87)90237-x.
The metC gene of Salmonella typhimurium was cloned into the plasmid vectors pACYC184 and pBR322. Genetic and biochemical experiments indicate that the region controlling metC gene expression is present on the cloned fragments. The location of the metC gene was determined by insertional inactivation with transposons Tn5 and mini-Mu. The gene product was identified in a minicell system as a 49-kDa polypeptide. The direction of transcription and translation was determined by correlating the orientation of mini-Mu insertions within the metC gene with the expression of the lacZ gene contained in mini-Mu.
鼠伤寒沙门氏菌的metC基因被克隆到质粒载体pACYC184和pBR322中。遗传和生化实验表明，控制metC基因表达的区域存在于克隆片段上。通过转座子Tn5和mini-Mu的插入失活确定了metC基因的位置。在一个小细胞系统中鉴定出该基因产物是一种49 kDa的多肽。通过将metC基因内mini-Mu插入的方向与mini-Mu中所含lacZ基因的表达相关联，确定了转录和翻译的方向。
