鼠伤寒沙门氏菌cysAMK区域的克隆与特性分析
Cloning and characterization of the cysAMK region of Salmonella typhimurium.
作者信息
Hulanicka M D, Garrett C, Jagura-Burdzy G, Kredich N M
出版信息
J Bacteriol. 1986 Oct;168(1):322-7. doi: 10.1128/jb.168.1.322-327.1986.
Abstract
A total of 30 kilobases of DNA comprising the cysAMK region of S. typhimurium was cloned as a series of fragments in phage lambda 1059. The genetic organization of this region was established through studies of gene expression from fragments subcloned in pBR322 and from blot hydridization analyses of restriction sites in chromosomal DNA from multisite deletion strains. The results give a gene order of cysA-cysM-crr-ptsl-ptsH-cysK over a distance of approximately 12 kilobases. cysM and cysA have been cloned and expressed in pBR322; attempts to obtain stable pBR322 derivatives carrying cysK were unsuccessful.
摘要
将包含鼠伤寒沙门氏菌cysAMK区域的总共30千碱基DNA作为一系列片段克隆到噬菌体λ1059中。通过对亚克隆到pBR322中的片段的基因表达以及对多位点缺失菌株染色体DNA中限制性位点的印迹杂交分析的研究，确定了该区域的遗传组织。结果给出了在大约12千碱基的距离上cysA - cysM - crr - ptsl - ptsH - cysK的基因顺序。cysM和cysA已被克隆并在pBR322中表达；获得携带cysK的稳定pBR322衍生物的尝试未成功。
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