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兔疱疹病毒DNA的克隆与物理图谱分析

Cloning and physical mapping of Herpesvirus sylvilagus DNA.

作者信息

Rouhandeh H, Cohrs R

机构信息

Department of Microbiology, Southern Illinois University, Carbondale 62901.

出版信息

Gene. 1987;61(1):31-9. doi: 10.1016/0378-1119(87)90362-3.

Abstract

The physical map positions for the EcoRI restriction fragments of Herpesvirus sylvilagus DNA were determined using cloned virus DNA fragments as probes for hybridization. Approximately 75% of the virus genome was cloned. The internal location of the repeated nucleotide sequences and the lack of isomeric forms suggested that the virus genome belongs to the class C herpesvirus DNA.

摘要

利用克隆的病毒DNA片段作为杂交探针,确定了兔疱疹病毒DNA的EcoRI限制性片段的物理图谱位置。大约75%的病毒基因组被克隆。重复核苷酸序列的内部位置以及缺乏异构体形式表明该病毒基因组属于C类疱疹病毒DNA。

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