Brooks Adam C, Hwang Ling Chin
Department of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Sheffield, United Kingdom.
Department of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Sheffield, United Kingdom.
Plasmid. 2017 May;91:37-41. doi: 10.1016/j.plasmid.2017.03.004. Epub 2017 Mar 18.
Bacterial plasmid and chromosome segregation systems ensure that genetic material is efficiently transmitted to progeny cells. Cell-based studies have shed light on the dynamic nature and the molecular basis of plasmid partition systems. In vitro reconstitutions, on the other hand, have proved to be an invaluable tool for studying the minimal components required to elucidate the mechanism of DNA segregation. This allows us to gain insight into the biological and biophysical processes that enable bacterial cells to move and position DNA. Here, we review the reconstitutions of plasmid partition systems in cell-free reactions, and discuss recent work that has begun to challenge long standing models of DNA segregation in bacteria.
细菌质粒和染色体分离系统确保遗传物质有效地传递给子代细胞。基于细胞的研究揭示了质粒分配系统的动态性质和分子基础。另一方面，体外重建已被证明是研究阐明DNA分离机制所需最小成分的宝贵工具。这使我们能够深入了解使细菌细胞移动和定位DNA的生物学和生物物理过程。在这里，我们回顾了无细胞反应中质粒分配系统的重建，并讨论了最近开始挑战细菌中DNA分离长期模型的工作。
