Soto Alberto, Gasalla Patricia, Begega Azucena, López Matías
Department of Psychology, University of Oviedo, Oviedo, Spain.
Department of Psychology, University of Oviedo, Oviedo, Spain.
Neurosci Lett. 2017 Apr 24;647:32-37. doi: 10.1016/j.neulet.2017.03.025. Epub 2017 Mar 18.
This study examined c-Fos expression in selected brain areas consequent to intraperitoneal (IP) administration of saccharin and lithium chloride. Rats were tested for aversion to the saccharin as measured by flavor consumption and orofacial reactions in the taste reactivity (TR) test. It was found that intraperitoneal conditioning resulted in the reduction in voluntary consumption but not in the production of aversive orofacial responses to the saccharin. The immunohistochemistry quantification revealed increased c-Fos activity in the insular cortex, the shell and core regions of the nucleus accumbens, and the basolateral nucleus of the amygdala. These results show that a conditioned taste aversion can be induced without direct oropharyngeal gustatory stimulation at the time of conditioning. In addition, this study provide evidence of increased neural activity in response to intraperitoneal saccharin injections.
本研究检测了腹腔注射糖精和氯化锂后，特定脑区中c-Fos的表达情况。通过味觉反应（TR）测试中的味道消耗量和口面部反应来检测大鼠对糖精的厌恶程度。结果发现，腹腔注射条件作用导致了自愿摄入量的减少，但并未产生对糖精的厌恶口面部反应。免疫组织化学定量分析显示，岛叶皮质、伏隔核的壳区和核心区以及杏仁核的基底外侧核中的c-Fos活性增加。这些结果表明，在条件作用时无需直接的口咽味觉刺激即可诱发条件性味觉厌恶。此外，本研究为腹腔注射糖精后神经活动增加提供了证据。
