Beckmann A M, Kiviat N B, Daling J R, Sherman K J, McDougall J K
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Int J Gynecol Pathol. 1988;7(1):39-47. doi: 10.1097/00004347-198803000-00004.
We examined lesions from six women with multifocal neoplasia of the lower genital tract (vulva and cervix or vulva and vagina) for the presence of human papillomavirus (HPV) DNA sequences by DNA-DNA in situ hybridization. Cervical tissue specimens from four of the five women with carcinoma in situ (CIS) of the cervix were found to contain HPV type 16 DNA sequences. Vulvar lesions from three of these women also contained HPV-16 DNA and were histologically consistent with CIS. In each instance, hybridization with HPV-16 DNA probes was seen only in areas of the epithelium that contained evidence of surface maturation or koilocytotic atypia (KA). The HPV DNA was not visualized in regions of these lesions in which the full thickness of the epithelium was occupied by poorly differentiated cells. Virus capsid antigens were only detected in a very few cells in two of the four cervical lesions that contained HPV-16 DNA. HPV type 16, which has consistently been associated with the development of cervical cancer, is further implicated as an agent in the pathogenesis of genital cancers by demonstration of the virus genome in neoplasia at nonadjacent sites.
我们通过DNA-DNA原位杂交检查了6名患有下生殖道（外阴和子宫颈或外阴和阴道）多灶性肿瘤形成的女性的病变组织，以检测人乳头瘤病毒（HPV）DNA序列。在5名患有子宫颈原位癌（CIS）的女性中，有4名的子宫颈组织标本被发现含有HPV 16型DNA序列。其中3名女性的外阴病变也含有HPV-16 DNA，并且在组织学上与CIS一致。在每种情况下，仅在含有表面成熟或空泡细胞异型性（KA）证据的上皮区域观察到与HPV-16 DNA探针的杂交。在这些病变的区域中，若上皮全层被低分化细胞占据，则未观察到HPV DNA。在4个含有HPV-16 DNA的子宫颈病变中，只有2个病变中的极少数细胞检测到病毒衣壳抗原。HPV 16型一直与子宫颈癌的发生相关，通过在非相邻部位的肿瘤中证实病毒基因组，进一步表明其在生殖器癌症发病机制中发挥作用。
