Roohie R Kaunain, Umesha S
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India.
3 Biotech. 2015 Dec;5(6):1089-1100. doi: 10.1007/s13205-015-0311-8. Epub 2015 Jun 23.
The suppression subtractive hybridization was employed to elucidate the resistance mechanism in Brassica oleracea var. capitata upon infection with Xanthomonas campestris pv. campestris. A cDNA library was constructed enriched in differentially expressed transcripts in the resistant cultivar Pusa mukta. A total of 150 unigenes were classified into five functional categories. The present study indicates that the defense-related unigenes accounted for the 35 % of the total unigenes studied. Confirmation of defense-specific representation genes through semiquantitative RT-PCR revealed their increased expression in the resistant cultivar which was validated by qPCR. The resistant cultivar elicited a strong hypersensitive response upon attack by black rot pathogen. The study is first of its kind where the resistant cultivar Pusa mukta in India has been assessed for its resistance to the bacterial pathogen.
采用抑制性消减杂交技术来阐明甘蓝型油菜在感染野油菜黄单胞菌野油菜致病变种后其抗逆机制。构建了一个富含抗性品种Pusa mukta中差异表达转录本的cDNA文库。总共150个单基因被分为五个功能类别。本研究表明，与防御相关的单基因占所研究单基因总数的35%。通过半定量RT-PCR对防御特异性代表性基因的验证表明，它们在抗性品种中的表达增加，这一点通过定量PCR得到了证实。抗性品种在受到黑腐病菌攻击时引发了强烈的过敏反应。该研究首次对印度的抗性品种Pusa mukta对细菌病原体的抗性进行了评估。
