Dilipkumar M, Rajasimman M, Rajamohan N
Department of Chemical Engineering, Annamalai University, Annamalainagar, Tamilnadu, 608002, India.
Department of Chemical Engineering, Sohar University, Sohar, 311, Oman.
3 Biotech. 2013 Dec;3(6):509-515. doi: 10.1007/s13205-012-0112-2. Epub 2013 Jan 3.
In this work, inulinase was produced by solid state fermentation by Streptomyces sp. using copra waste as carbon source. The nutrients were screened by Plackett-Burman design. From the pareto chart it was found that the nutrients, namely, soya bean cake, MgSO·7HO and (NH)SO were found to be most significant nutrient components. Hence, these three components were selected for further optimization using central composite design (CCD) in response surface methodology (RSM). The optimum conditions were soya bean cake: 0.05711 g/gds, MgSO·7HO: 0.00063 g/gds and (NH)SO: 0.00772 g/gds. Under these optimized conditions, the production of inulinase was found to be 131 U/gds. The constants in the Michaelis-Menten equation were evaluated and high R value implies the fitness of the model.
在这项工作中，链霉菌属通过固态发酵利用椰干废料作为碳源生产菊粉酶。采用Plackett-Burman设计对营养成分进行筛选。从帕累托图中发现，营养成分大豆饼、MgSO·7H₂O和(NH₄)₂SO₄是最显著的营养成分。因此，选择这三种成分使用响应面法（RSM）中的中心复合设计（CCD）进行进一步优化。最佳条件为大豆饼：0.05711 g/gds，MgSO·7H₂O：0.00063 g/gds，(NH₄)₂SO₄：0.00772 g/gds。在这些优化条件下，菊粉酶的产量为131 U/gds。评估了米氏方程中的常数，高R值表明模型的拟合度。
