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利用统计设计对链霉菌在固态发酵中从大蒜生产菊粉酶的工艺进行优化

Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs.

作者信息

Dilipkumar M, Rajasimman M, Rajamohan N

机构信息

Department of Chemical Engineering, Annamalai University, Annamalainagar, Tamilnadu 608002, India.

出版信息

Biotechnol Res Int. 2011 Mar 30;2011:708043. doi: 10.4061/2011/708043.

DOI:10.4061/2011/708043
PMID:21541216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3085327/
Abstract

Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH(4)NO(3), MnSO(4)·7H(2)O, Soya bean cake, and K(2)HPO(4) were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH(4)NO(3)-6.63 mg/gds, MnSO(4)·7H(2)O-26.16 mg/gds, Soya bean cake-60.6 mg/gds, and K(2)HPO(4)-5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.

摘要

采用Plackett-Burman设计筛选了18种营养成分，用于链霉菌以大蒜为底物固态发酵生产菊粉酶。通过实验发现，4种营养成分，即硝酸铵、七水硫酸锰、豆饼和磷酸氢二钾是最显著的营养成分。因此，选择了这4种成分。使用响应面法（RSM）对所选成分进行了优化。最佳条件为硝酸铵-6.63毫克/克干基、七水硫酸锰-26.16毫克/克干基、豆饼-60.6毫克/克干基和磷酸氢二钾-5.24毫克/克干基。在这些条件下，菊粉酶的产量为76单位/克干基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/d166072284ef/BTRI2011-708043.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/b33d5f56ecd1/BTRI2011-708043.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/3d3719f425ba/BTRI2011-708043.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/a68ca8848e00/BTRI2011-708043.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/d166072284ef/BTRI2011-708043.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/b33d5f56ecd1/BTRI2011-708043.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/3d3719f425ba/BTRI2011-708043.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/a68ca8848e00/BTRI2011-708043.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6572/3085327/d166072284ef/BTRI2011-708043.004.jpg

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