Dhanapal Arun Prabhu, Crisosto Carlos H
Department of Plant Sciences, University of California Davis, One Shields Ave, Davis, CA, 95616, USA.
Division of Plant Sciences, University of Missouri, 1-31 Agricultural Building, Columbia, MO, 65211, USA.
3 Biotech. 2013 Dec;3(6):481-490. doi: 10.1007/s13205-012-0109-x. Epub 2013 Jan 5.
Peach and nectarine (Prunus persica L.) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0-5 °C) is a common strategy used to slow the ripening processes and to extend shelf life. However, if susceptible varieties are held too long at a low temperature, they will not ripen properly and will develop chilling injury (CI) symptoms like mealiness (M), flesh browning (FB), and flesh bleeding (FBL). Understanding the genetic control of these traits to produce CI resistant cultivars will greatly benefit producers, shippers and consumers. In this study, we evaluated a population of 51 individuals from Pop-DG across 4 years with CI traits observed in one or two time points to detect molecular marker association with selected 960 single-nucleotide polymorphisms (SNPs) from 1,536 SNPs chip. Genotypic and phenotypic data were analyzed by general linear model and mixed linear model to see comparative results from both analyses. Among 960 SNPs used, 22 SNPs were found associated with CI susceptibility traits like M, FB, and FBL. Many SNP markers were located in or close to previously reported quantitative trait loci mapped by linkage analysis.
桃和油桃（Prunus persica L.）极易腐烂，在常温下会迅速成熟和变质。低温（0-5°C）贮藏是延缓成熟过程和延长货架期的常用策略。然而，如果易感品种在低温下贮藏时间过长，它们将无法正常成熟，并会出现冷害（CI）症状，如粉质化（M）、果肉褐变（FB）和果肉出血（FBL）。了解这些性状的遗传控制以培育抗冷害品种将极大地造福生产者、运输商和消费者。在本研究中，我们对Pop-DG群体中的51个个体进行了为期4年的评估，在一到两个时间点观察冷害性状，以检测与从1536个SNP芯片中选择的960个单核苷酸多态性（SNP）的分子标记关联。通过一般线性模型和混合线性模型分析基因型和表型数据，以查看两种分析的比较结果。在使用的960个SNP中，发现22个SNP与粉质化、果肉褐变和果肉出血等冷害易感性状相关。许多SNP标记位于通过连锁分析绘制的先前报道的数量性状位点内或附近。
