Su Yao-Chi, Yu Chang-you, Lin Jiang-Liang, Lai Jyh-mirn, Chen Shu-Wun, Tu Pei-Chun, Chu Chishih
Department of Veterinary Medicine, National Chiayi University, 300 University Road, Chiayi City 60004, Taiwan, Republic of China.
Avian Dis. 2011 Jun;55(2):217-22. doi: 10.1637/9420-060910-Reg.1.
Salmonellosis is a common food-borne illness in humans caused by Salmonella-contaminated poultry and their products. In hatcheries, 110 Salmonella isolates were identified, mostly from first enrichment, and few from delayed enrichment. The Salmonella prevalence in goose and duck hatcheries was higher when measured by four multiplex PCR methods than by traditional culture (73.8% vs. 44.35%, P < 0.05); 97.3% of 110 isolates were Salmonella Potsdam of serogroup C1 and other isolates were Salmonella Montevideo of C1 and Salmonella Albany of C2. Plasmid and pulsed field gel electrophoresis genetic analysis revealed that isolates from duck hatcheries were more diverse than those from goose hatcheries. In Salmonella Potsdam, host species-specific genotypes were observed in geese for genotypes 3, 4, and 5 and in ducks for genotypes 7, 8, and 9, suggesting that Salmonella Potsdam may evolve into goose- and duck-specific isolates. An examination of 1121 eggs found that only Salmonella Potsdam was identified in 1.8% (7/591) of eggs from chickens fed on the ground, not housed in cages, and in egg content (6/7) as well as eggshell membrane (1/7). In conclusion, Salmonella Potsdam may be a major Salmonella infection in waterfowl and chicken eggs.
沙门氏菌病是一种常见的食源性人类疾病，由受沙门氏菌污染的家禽及其产品引起。在孵化场，共鉴定出110株沙门氏菌分离株，大多来自初次富集培养，少数来自延迟富集培养。用四种多重PCR方法检测时，鹅和鸭孵化场的沙门氏菌患病率高于传统培养法（73.8%对44.35%，P<0.05）；110株分离株中97.3%为C1血清群的波茨坦沙门氏菌，其他分离株为C1血清群的蒙得维的亚沙门氏菌和C2血清群的奥尔巴尼沙门氏菌。质粒和脉冲场凝胶电泳基因分析表明，鸭孵化场的分离株比鹅孵化场的分离株更多样化。在波茨坦沙门氏菌中，在鹅中观察到3、4和5型宿主物种特异性基因型，在鸭中观察到7、8和9型，这表明波茨坦沙门氏菌可能演变成鹅和鸭特异性分离株。对1121枚鸡蛋的检测发现，在地面饲养而非笼养的鸡所产鸡蛋中，仅在1.8%（7/591）的鸡蛋中检出波茨坦沙门氏菌，在蛋清（6/7）和蛋壳膜（1/7）中也有检出。总之，波茨坦沙门氏菌可能是水禽和鸡蛋中主要的沙门氏菌感染源。
