基于光片荧光显微镜（LSFM）的丝状真菌三维实时成像

Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM).

作者信息

Pampaloni Francesco, Knuppertz Laura, Hamann Andrea, Osiewacz Heinz D, Stelzer Ernst H K

机构信息

Physical Biology Group, Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt am Main, Max-von-Laue-Str. 15, D-60438, Frankfurt am Main, Germany.

Institute of Molecular Biosciences and Cluster of Excellence Frankfurt Macromolecular Complexes, Department of Biosciences, Goethe Universität Frankfurt am Main, Max-von-Laue-Str. 9, 60438, Frankfurt am Main, Germany.

出版信息

Methods Mol Biol. 2017;1563:19-31. doi: 10.1007/978-1-4939-6810-7_2.

DOI:10.1007/978-1-4939-6810-7_2

PMID:28324599

Abstract

We describe a method for the three-dimensional live imaging of filamentous fungi with light sheet-based fluorescence microscopy (LSFM). LSFM provides completely new opportunities to investigate the biology of fungal cells and other microorganisms with high spatial and temporal resolution. As an example, we study the established aging model Podospora anserina. The protocol explains the mounting of the live fungi for the light sheet imaging, the imaging procedure and illustrates basic image processing of data.

摘要

我们描述了一种使用基于光片的荧光显微镜（LSFM）对丝状真菌进行三维实时成像的方法。LSFM为以高空间和时间分辨率研究真菌细胞及其他微生物的生物学特性提供了全新的机会。例如，我们研究了已建立的衰老模型——嗜热栖热放线菌。该方案解释了用于光片成像的活真菌的固定方法、成像过程，并展示了数据的基本图像处理。