细胞生物学中的光片显微镜技术。

Light sheet microscopy in cell biology.

作者信息

Tomer Raju, Khairy Khaled, Keller Philipp J

机构信息

Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

出版信息

Methods Mol Biol. 2013;931:123-37. doi: 10.1007/978-1-62703-056-4_7.

DOI:10.1007/978-1-62703-056-4_7

PMID:23027001

Abstract

Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth. This unique combination of capabilities makes light sheet-based microscopes highly suitable for live imaging applications. Here, we provide an overview of light sheet-based microscopy assays for in vitro and in vivo imaging of biological samples, including cell extracts, soft gels, and large multicellular organisms. We furthermore describe computational tools for basic image processing and data inspection.

摘要

基于光片的荧光显微镜技术（LSFM）正成为生命科学领域一种强大的成像技术。LSFM具有极高的成像速度、高信噪比、低光漂白水平以及良好的光学穿透深度。这些独特的性能组合使得基于光片的显微镜非常适合用于活细胞成像应用。在这里，我们概述了用于生物样品体外和体内成像的基于光片的显微镜检测方法，包括细胞提取物、软凝胶和大型多细胞生物体。我们还描述了用于基本图像处理和数据检查的计算工具。