Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School , Boston, Massachusetts 02115, United States.
Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin , Madison, Wisconsin 53705, United States.
Org Lett. 2017 Apr 7;19(7):1772-1775. doi: 10.1021/acs.orglett.7b00545. Epub 2017 Mar 22.
Three new dentigerumycin analogues are produced by Streptomyces sp. M41, a bacterium isolated from a South African termite, Macrotermes natalensis. The structures of the complex nonribosomal peptide synthetase-polyketide synthase (NRPS/PKS) hybrid compounds were determined by 1D- and 2D-NMR spectroscopy, high-resolution mass spectrometry, and circular dichroism (CD) spectroscopy. Both cyclic and linear peptides are reported, and the genetic organization of the NRPS modules within the biosynthetic gene cluster accounts for the observed structural diversity.
三种新的齿霉素类似物由从南非白蚁 Macrotermes natalensis 中分离出的细菌 Streptomyces sp. M41 产生。通过 1D 和 2D-NMR 光谱、高分辨率质谱和圆二色性 (CD) 光谱确定了复杂的非核糖体肽合酶-聚酮合酶 (NRPS/PKS) 杂合化合物的结构。报告了既有的环状肽和线性肽，并且生物合成基因簇中 NRPS 模块的遗传组织解释了观察到的结构多样性。
