Cereda P M, Debiaggi M, Mignatti P, Pagani L, Romero E
Microbiologica. 1984 Jul;7(3):251-6.
We have obtained a new cell line persistently infected with bovine leukemia virus (BLV). BLV-infected cells can be easily detected by indirect immunofluorescence or immunoperoxidase staining technique. The morphology and number of infected cells seem to be related to serum concentration in culture medium: fetal calf serum (FCS) showed a marked effect on virus growth and virus distribution in cell cultures. All of the commercial lots of FCS tested showed BLV inhibiting activity very similar to that previously described for other viruses.
我们获得了一种持续感染牛白血病病毒(BLV)的新细胞系。通过间接免疫荧光或免疫过氧化物酶染色技术可轻松检测出被BLV感染的细胞。感染细胞的形态和数量似乎与培养基中的血清浓度有关:胎牛血清(FCS)对病毒在细胞培养物中的生长和分布有显著影响。所测试的所有市售批次FCS均显示出与先前针对其他病毒所描述的非常相似的BLV抑制活性。