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人血小板衍生生长因子A链cDNA和基因的结构特征：外显子的选择性使用预示着两种不同的前体蛋白。

Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.

作者信息

Rorsman F, Bywater M, Knott T J, Scott J, Betsholtz C

机构信息

Department of Pathology, University Hospital, Uppsala, Sweden.

出版信息

Mol Cell Biol. 1988 Feb;8(2):571-7. doi: 10.1128/mcb.8.2.571-577.1988.

DOI:10.1128/mcb.8.2.571-577.1988
PMID:2832727
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363182/
Abstract

The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons was determined. Seven exons were identified, spanning approximately 22 kilobase pairs of genomic DNA. Alternative exon usage, identified by cDNA cloning, occurs in a human glioblastoma cell line and may give rise to two types of A-chain precursors with different C termini. The exon-intron arrangement was similar to that of the PDGF B-chain/sis locus and seemed to divide the precursor proteins into functional domains. Southern blot analysis of genomic DNA showed that a single PDGF A-chain gene was present in the human genome.

摘要

通过限制性内切酶分析对人血小板衍生生长因子（PDGF）A链基因座进行了表征，并确定了其外显子的核苷酸序列。鉴定出七个外显子，跨越约22千碱基对的基因组DNA。通过cDNA克隆鉴定出的外显子选择性使用发生在人胶质母细胞瘤细胞系中，可能产生具有不同C末端的两种类型的A链前体。外显子-内含子排列与PDGF B链/ sis基因座相似，似乎将前体蛋白分为功能域。基因组DNA的Southern印迹分析表明，人类基因组中存在单个PDGF A链基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20fc/363182/30975dffc4dd/molcellb00062-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20fc/363182/30975dffc4dd/molcellb00062-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20fc/363182/30975dffc4dd/molcellb00062-0070-a.jpg

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