Matos-Rocha Thiago José, Cavalcanti Marília Gabriela Dos Santos, Barbosa-Filho José Maria, Lúcio Ana Silvia Suassuna Carneiro, Veras Dyana Leal, Marques Márcia Ortiz Mayo, Alves Luiz Carlos, Brayner Fábio André
Centro de Pesquisas Aggeu Magalhães, Departamento de Parasitologia, Laboratório de Biologia Celular e Molecular, Recife, PE, Brasil.
Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami, Recife, PE, Brasil.
Rev Soc Bras Med Trop. 2017 Jan-Feb;50(1):86-91. doi: 10.1590/0037-8682-0285-2016.
: Schistosomiasis, a parasitic disease caused by trematode flatworms of the genus Schistosoma, affects more than 200 million people worldwide, and its control is dependent on a single drug, praziquantel. Here, we report the in vitro effect of rotundifolone, a monoterpene isolated from Mentha x villosa (Lamiaceae), on Schistosoma mansoni adult worms.
: The in vitro effect of rotundifolone on adult Schistosoma mansoni was evaluated by analysis of behavior and mortality and through a scanning electron microscopic analysis of ultrastructural changes in the tegument of the worms.
: At concentrations of 3.54 and 7.09μg/mL-1 rotundifolone, no worm mortality was observed at any of the sampling intervals. A minor reduction in movement of the tail, suckers, and gynecophoral canal membrane was observed after 96 h of exposure to 7.09μg/mL-1 rotundifolone. At 70.96μg/mL-1, a lack of movement was observed from 72h onwards and all worms were deemed dead; similar effects were observed at 48h with 177.4μg/mL-1, and at 24h with 354.8μg/mL-1 and 700.96μg/mL-1. Rotundifolone also caused death of all parasites and separation of coupled pairs into individual males and females after 24h at 354.8μg/mL-1.
: The main changes in the tegument induced by the different ROT treatments were: after 24h incubation, bubble lesions spread over the entire body and loss of tubercles occurred in some regions of the ventral region.
血吸虫病是一种由血吸虫属吸虫纲扁虫引起的寄生虫病，全球有超过2亿人受其影响，其防治依赖于单一药物吡喹酮。在此，我们报告了从绒毛薄荷（唇形科）中分离出的单萜类化合物圆叶当归酮对曼氏血吸虫成虫的体外作用。
通过行为和死亡率分析以及对虫体体表超微结构变化的扫描电子显微镜分析，评估圆叶当归酮对曼氏血吸虫成虫的体外作用。
在圆叶当归酮浓度为3.54和7.09μg/mL-1时，在任何采样间隔均未观察到虫体死亡。暴露于7.09μg/mL-1圆叶当归酮96小时后，观察到尾部、吸盘和抱雌沟膜的运动略有减少。在70.96μg/mL-1时，从72小时起观察到虫体不动，所有虫体均被视为死亡；在48小时时，177.4μg/mL-1的浓度下观察到类似效果，在24小时时，354.8μg/mL-1和700.96μg/mL-1的浓度下也观察到类似效果。在354.8μg/mL-1的浓度下，24小时后圆叶当归酮还导致所有寄生虫死亡，并使配对的雌雄虫体分离为单个雄虫和雌虫。
不同圆叶当归酮处理诱导的体表主要变化为：孵育24小时后，气泡样病变遍布全身，腹部区域某些部位的结节消失。
