Molecular and Cell Biology Laboratory, Department of Parasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil.
Planta Med. 2013 Sep;79(14):1307-12. doi: 10.1055/s-0033-1350732. Epub 2013 Aug 14.
This study aimed to determine the composition of the essential oil of Mentha x villosa and to evaluate its biological effects in vitro on adult worms of S. mansoni. Rotundifolone (70.96 %), limonene (8.75 %), trans-caryophyllene (1.46 %), and β-pinene (0.81 %) were shown to be the major constituents of this oil. Adult worms of S. mansoni were incubated with different concentrations of the essential oil (1, 10, 100, 250, 500, and 1000 µg/mL) and of its constituents rotundifolone (0.7, 3.54, 7.09, 70.96, 177.4, 354.8, and 700.96 µg/mL), limonene (43.75 µg/mL), trans-caryophyllene (7.3 µg/mL), and β-pinene (4.03 µg/mL). No schistosomicidal activity was identified at the trans-caryophyllene and β-pinene concentrations studied. However, use of the essential oil (10 µg/mL), rotundifolone (7.09 µg/mL), and limonene (43.75 µg/mL) resulted in decreased worm motility continuing until 96 hours of observation. At higher concentrations (100 and 70.96 µg/mL, respectively), both the essential oil and rotundifolone caused mortality among adult worms of S. mansoni. The positive control praziquantel caused the death of all parasites after 24 h of evaluation. The results from this study suggest that the essential oil of Mentha x villosa presents schistosomicidal efficacy.
本研究旨在确定绵毛水苏精油的成分，并评估其对曼氏血吸虫成虫的体外生物学效应。该油的主要成分分别为旋覆花酮（70.96%）、柠檬烯（8.75%）、反式石竹烯（1.46%）和β-蒎烯（0.81%）。将不同浓度的精油（1、10、100、250、500 和 1000μg/mL）及其成分旋覆花酮（0.7、3.54、7.09、70.96、177.4、354.8 和 700.96μg/mL）、柠檬烯（43.75μg/mL）、反式石竹烯（7.3μg/mL）和β-蒎烯（4.03μg/mL）与曼氏血吸虫成虫孵育。在所研究的反式石竹烯和β-蒎烯浓度下，未发现杀血吸虫活性。然而，使用精油（10μg/mL）、旋覆花酮（7.09μg/mL）和柠檬烯（43.75μg/mL）可降低虫体的运动性，持续至 96 小时观察期结束。在更高的浓度（分别为 100 和 70.96μg/mL）下，精油和旋覆花酮均导致曼氏血吸虫成虫死亡。阳性对照药吡喹酮在评价 24 小时后导致所有寄生虫死亡。本研究结果表明，绵毛水苏精油具有杀血吸虫功效。
