VANE J R
Br J Pharmacol Chemother. 1964 Oct;23(2):360-73. doi: 10.1111/j.1476-5381.1964.tb01592.x.
A method is described for the assay of circulating hormones after their injection or release into the circulation. The method is applicable to cats, dogs and rabbits, and consists of bathing or superfusing isolated smooth muscle preparations in a stream of heparinized arterial blood taken from and returned to the animal at a constant rate. The tone of the smooth muscle preparations was affected by small changes in the concentrations of various amines. Thus increases in blood concentrations of catechol amines can be assayed with the rat stomach strip and chick rectum preparations. The proportions of adrenaline and noradrenaline in a mixture can be determined. Circulating histamine can be assayed on the blood-bathed guinea-pig ileum and bradykinin on the rat duodenum preparations. The uses and limitations of the technique are discussed.
本文描述了一种在激素注射或释放进入循环系统后测定循环激素的方法。该方法适用于猫、狗和兔子，具体操作是将取自动物并以恒定速率回输至动物体内的肝素化动脉血以液流形式灌注或 superfusing 分离的平滑肌制剂。平滑肌制剂的张力会受到各种胺类浓度微小变化的影响。因此，儿茶酚胺血浓度的升高可用大鼠胃条和鸡直肠制剂进行测定。混合物中肾上腺素和去甲肾上腺素的比例可以确定。循环组胺可用血浴豚鼠回肠进行测定，缓激肽可用大鼠十二指肠制剂进行测定。文中还讨论了该技术的用途和局限性。 （注：“superfusing”这个词原文有误，推测可能是“superfusing”，意为“灌注”，这里按此推测翻译，若实际另有正确词汇，请根据正确词汇调整）
