Ajayi Abayomi M, Ologe Mary O, Ben-Azu Benneth, Okhale Samuel E, Adzu Bulus, Ademowo Olusegun G
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J Basic Clin Physiol Pharmacol. 2017 Nov 27;28(6):531-541. doi: 10.1515/jbcpp-2016-0096.
Ocimum gratissimum leaf is used in managing rheumatism and other inflammatory conditions. In this study, we investigated the antioxidant and anti-inflammatory effects of phenolic extract obtained by sequential methanol extraction of O. gratissimum leaves (MEOg).
The methanol extract (MEOg) was obtained after sequential maceration (n-hexane, chloroform and methanol) of dried O. gratissimum leaves. The fingerprint of the extract was obtained using a high-performance liquid chromatrographic method. In vitro effects were tested by 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), nitric oxide (NO) free radical scavenging, lipoxygenase, and xanthine oxidase inhibitory assays. MEOg was studied for anti-inflammatory activity in carrageenan-induced paw edema and air pouch inflammation in rats.
HPLC fingerprint of the extract shows the presence of caffeic acid, rutin, ferulic acid, apigenin, and quercetin. Antioxidant activity of MEOg revealed an IC50 value in DPPH (31.5±0.03 μg/mL) and NO assay (201.6±0.01 μg/mL), respectively. The extract demonstrated strong xanthine oxidase inhibitory and weak antilipoxygenase activities. MEOg (100 mg/kg) significantly inhibited carrageenan-induced paw edema by 43.2%. Furthermore, MEOg (50 and 100 mg/kg) significantly reduced exudate volume, leucocyte count, neutrophil infiltration, TNF-α, nitrites, myeloperoxidase, and malondialdehyde in carrageenan-induced air pouch inflammation. MEOg also elevated the glutathione levels in the inflammatory exudates.
MEOg shows potential therapeutic benefits in slowing down inflammation and oxidative stress in chronic diseases, such as arthritis.
丁香罗勒叶用于治疗风湿病和其他炎症性疾病。在本研究中，我们调查了通过顺序甲醇提取丁香罗勒叶获得的酚类提取物（MEOg）的抗氧化和抗炎作用。
干燥的丁香罗勒叶经顺序浸渍（正己烷、氯仿和甲醇）后获得甲醇提取物（MEOg）。采用高效液相色谱法获得提取物的指纹图谱。通过1,1-二苯基-2-苦基肼（DPPH）、一氧化氮（NO）自由基清除、脂氧合酶和黄嘌呤氧化酶抑制试验检测体外效果。研究了MEOg对角叉菜胶诱导的大鼠爪肿胀和气囊炎症的抗炎活性。
提取物的HPLC指纹图谱显示存在咖啡酸、芦丁、阿魏酸、芹菜素和槲皮素。MEOg的抗氧化活性在DPPH试验（31.5±0.03μg/mL）和NO试验（201.6±0.01μg/mL）中分别显示出IC50值。该提取物表现出较强的黄嘌呤氧化酶抑制活性和较弱的抗脂氧合酶活性。MEOg（100mg/kg）显著抑制角叉菜胶诱导的爪肿胀43.2%。此外，MEOg（50和100mg/kg）显著降低角叉菜胶诱导的气囊炎症中的渗出液体积、白细胞计数、中性粒细胞浸润、TNF-α、亚硝酸盐、髓过氧化物酶和丙二醛。MEOg还提高了炎症渗出液中的谷胱甘肽水平。
MEOg在减缓慢性疾病如关节炎的炎症和氧化应激方面显示出潜在的治疗益处。
